Recombinant Human WEE1 Protein (215-646, end), N-FLAG tagged

• Cat.No.: WEE1-14H
• Product Overview: Human Wee1 protein, also known as Wee1A Kinase, GenBank Accession No. NM_003390, amino acids 215-646 (end), with an N-terminal FLAG-tag, expressed in a Baculovirus infected Sf9 cell expression system.

Specification

• Description: This gene encodes a nuclear protein, which is a tyrosine kinase belonging to the Ser/Thr family of protein kinases. This protein catalyzes the inhibitory tyrosine phosphorylation of CDC2/cyclin B kinase, and appears to coordinate the transition between DNA replication and mitosis by protecting the nucleus from cytoplasmically activated CDC2 kinase.
• Source: Insect cells
• Species: Human
• Tag: Flag
• Form: Aqueous buffer solution
• Molecular Mass: 50 kDa
• Protein length: 215-646
• Storage: At least 6 months at –80 centigrade.
• Storage Buffer: 40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 100 μg/mL FLAG peptide and 20 % Glycerol.
• Shipping: At -80 centigrade
• Warning: Avoid freeze/thaw cycles. Protein may be diluted to ≥ 100 μg/ml in PBS + glycerol and stored at -80 centigrade.

Gene Information

• Gene Name: WEE1 WEE1 G2 checkpoint kinase [ Homo sapiens (human) ]
• Official Symbol: WEE1
• Synonyms: WEE1; WEE1 G2 checkpoint kinase; WEE1A; WEE1hu; wee1-like protein kinase; WEE1 homolog; WEE1+ homolog; protein kinase; wee1A kinase; EC 2.7.10.2
• Gene ID: 7465
• mRNA Refseq: NM_003390
• Protein Refseq: NP_003381
• MIM: 193525
• UniProt ID: P30291

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Q&As

How does WEE1 contribute to DNA damage response?

It plays a role in DNA damage response by delaying cell cycle progression to allow DNA repair.

What is the impact of altered WEE1 activity on cancer development?

Altered WEE1 activity is associated with the progression of various cancers.

What potential therapeutic applications arise from targeting WEE1 in cancer treatment?

Targeting WEE1 in cancer therapy could exploit its role in cell cycle regulation to inhibit tumor growth.

How do genetic variations in WEE1 affect cell division?

Genetic mutations in WEE1 can lead to uncontrolled cell division and genomic instability.

What role does WEE1 play in checkpoint regulation?

WEE1 is critical in checkpoint regulation, ensuring cells do not divide with damaged DNA.

How does WEE1 interact with other cell cycle proteins?

WEE1 works in conjunction with other cell cycle proteins to regulate the timing of cell division.

What is the primary role of WEE1 in cell cycle control?

WEE1 is a kinase that inhibits cell cycle progression, particularly at the G2/M checkpoint.