Recombinant Human WEE1 Protein (M291-K575), His tagged

• Cat.No.: WEE1-0060H
• Product Overview: Recombinant Human 8His-TEV-(G)-Wee1(M291-K575) Protein was expressed in Insect cell.

Specification

• Species: Human
• Source: Insect Cells
• Tag: His
• Protein Length: M291-K575
• Description: Acts as a negative regulator of entry into mitosis (G2 to M transition) by protecting the nucleus from cytoplasmically activated cyclin B1-complexed CDK1 before the onset of mitosis by mediating phosphorylation of CDK1 on 'Tyr-15'. Specifically phosphorylates and inactivates cyclin B1-complexed CDK1 reaching a maximum during G2 phase and a minimum as cells enter M phase. Phosphorylation of cyclin B1-CDK1 occurs exclusively on 'Tyr-15' and phosphorylation of monomeric CDK1 does not occur. Its activity increases during S and G2 phases and decreases at M phase when it is hyperphosphorylated. A correlated decrease in protein level occurs at M/G1 phase, probably due to its degradation.
• Form: Liquid
• Endotoxin: < 0.01 EU per μg of the protein
• Purity: 90%
• Stability: Samples are stable for up to twelve months from date of receipt at -20 to -80 centigrade.
• Storage: Store it under sterile conditions at -20 to -80 centigrade. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Storage Buffer: Supplied as sterile 50 mM Tris-HCl (pH7.5), 200 mM NaCl, 20% glycerol
• Shipping: It is shipped out with blue ice.

Gene Information

• Gene Name: WEE1 WEE1 homolog (S. pombe) [ Homo sapiens (human) ]
• Official Symbol: WEE1
• Synonyms: WEE1; WEE1 homolog (S. pombe); wee1+ (S. pombe) homolog; wee1-like protein kinase; wee1A kinase; WEE1+ homolog; WEE1A; WEE1hu; FLJ16446; DKFZp686I18166
• Gene ID: 7465
• mRNA Refseq: NM_001143976
• Protein Refseq: NP_001137448
• MIM: 193525
• UniProt ID: P30291