Recombinant Mouse Wee1 Protein, Myc/DDK-tagged
Cat.No. : | Wee1-6992M |
Product Overview : | Purified recombinant protein of Mouse WEE 1 homolog 1 (S. pombe) (Wee1), with C-terminal MYC/DDK tag, expressed in HEK293T cells. |
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Species : | Mouse |
Source : | HEK293 |
Tag : | DDK&Myc |
Description : | Acts as a negative regulator of entry into mitosis (G2 to M transition) by protecting the nucleus from cytoplasmically activated cyclin B1-complexed CDK1 before the onset of mitosis by mediating phosphorylation of CDK1 on 'Tyr-15'. Specifically phosphorylates and inactivates cyclin B1-complexed CDK1 reaching a maximum during G2 phase and a minimum as cells enter M phase. Phosphorylation of cyclin B1-CDK1 occurs exclusively on 'Tyr-15' and phosphorylation of monomeric CDK1 does not occur. Its activity increases during S and G2 phases and decreases at M phase when it is hyperphosphorylated. A correlated decrease in protein level occurs at M/G1 phase, probably due to its degradation. |
Molecular Mass : | 71.6 kDa |
Purity : | > 80% as determined by SDS-PAGE and Coomassie blue staining |
Stability : | Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles. |
Storage : | Store at -80 centigrade after receiving vials. |
Concentration : | >50 μg/mL as determined by microplate BCA method |
Storage Buffer : | 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol. |
Gene Name | Wee1 WEE 1 homolog 1 (S. pombe) [ Mus musculus (house mouse) ] |
Official Symbol | Wee1 |
Synonyms | WEE1; WEE 1 homolog 1 (S. pombe); wee1-like protein kinase; wee1A kinase; WEE1 tyrosine kinase; Wee1A |
Gene ID | 22390 |
mRNA Refseq | NM_009516 |
Protein Refseq | NP_033542 |
UniProt ID | P47810 |
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◆ Cell & Tissue Lysates | ||
WEE1-1930HCL | Recombinant Human WEE1 cell lysate | +Inquiry |
Not For Human Consumption!
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