Recombinant Human VDR protein, His-tagged

• Cat.No.: VDR-562H
• Product Overview: Recombinant Human VDR (P11473) aa. (Met272~Ser427) fused with His tag was produced in E. coli cells.

Specification

• Description: This gene encodes the nuclear hormone receptor for vitamin D3. This receptor also functions as a receptor for the secondary bile acid lithocholic acid. The receptor belongs to the family of trans-acting transcriptional regulatory factors and shows sequence similarity to the steroid and thyroid hormone receptors. Downstream targets of this nuclear hormone receptor are principally involved in mineral metabolism though the receptor regulates a variety of other metabolic pathways, such as those involved in the immune response and cancer. Mutations in this gene are associated with type II vitamin D-resistant rickets. A single nucleotide polymorphism in the initiation codon results in an alternate translation start site three codons downstream. Alternative splicing results in multiple transcript variants encoding different proteins.
• Source: E. coli
• Species: Human
• Tag: His
• Form: Freeze-dried powder
• Molecular Mass: Predicted Molecular Mass: 21.6kDa; Accurate Molecular Mass: 20kDa as determined by SDS-PAGE reducing conditions.
• Protein length: Met272~Ser427
• Purity: >92%
• Characteristic: Isoelectric point is 6.5.
• Applications: Cell culture; Activity Assays.
• Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
• Storage: Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
• Storage buffer: 100mM NaHCO3, 500mM NaCl, pH8.3, containing 0.01% sarcosyl and 5% Trehalose.
• Reconstitution: Reconstitute in 100mM NaHCO3, 500mM NaCl (pH8.3) to a concentration of 0.1-1.0 mg/mL. Do not vortex

Gene Information

• Gene Name: VDR vitamin D (1,25- dihydroxyvitamin D3) receptor [ Homo sapiens (human) ]
• Official Symbol: VDR
• Synonyms: VDR; vitamin D (1,25- dihydroxyvitamin D3) receptor; NR1I1; PPP1R163; vitamin D3 receptor; 1,25-dihydroxyvitamin D3 receptor; nuclear receptor subfamily 1 group I member 1; protein phosphatase 1, regulatory subunit 163; vitamin D nuclear receptor variant 1; vitamin D receptor
• Gene ID: 7421
• mRNA Refseq: NM_000376.2
• Protein Refseq: NP_000367.1
• UniProt ID: P11473