Recombinant Human Ubiquitin-Conjugating Enzyme E2M, His-tagged

Description :	UBE2M belongs to the ubiquitin-conjugating enzyme family. The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s.
Source :	E. coli
Species :	Human
Tag :	His
Form :	Liquid. 20mM Tris-HCl buffer (pH8.0) containing 20% glycerol, 0.2M NaCl, 1mM DTT.
Molecular Mass :	23.5 kDa (207aa) confirmed by MALDI-TOF
Protein length :	1-183aa
AA Sequence :	MGSSHHHHHH SSGLVPRGSH MGSHMIKLFS LKQQKKEEES AGGTKGSSKK ASAAQLRIQK DINELNLPKT CDISFSDPDD LLNFKLVICP DEGFYKSGKF VFSFKVGQGY PHDPPKVKCE TMVYHPNIDL EGNVCLNILR EDWKPVLTIN SIIYGLQYLF LEPNPEDPLN KEAAEVLQNN RRLFEQNVQR SMRGGYIGST YFERCLK
Purity :	>95% by SDS - PAGE
Applications :	SDS-PAGE
Storage :	Can be stored at 4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C. Avoid repeated freezing and thawing cycles.
Concentration :	1 mg/ml (determined by Bradford assay)

Gene Name	UBE2M ubiquitin-conjugating enzyme E2M [ Homo sapiens ]
Official Symbol	UBE2M
Synonyms	UBE2M; ubiquitin-conjugating enzyme E2M; ubiquitin conjugating enzyme E2M (homologous to yeast UBC12) , ubiquitin conjugating enzyme E2M (UBC12 homolog, yeast); NEDD8-conjugating enzyme Ubc12; hUbc12; UBC12; yeast UBC12 homolog; NEDD8 protein ligase; NEDD8 carrier protein; ubiquitin-protein ligase M; ubiquitin carrier protein M; ubiquitin-conjugating enzyme E2M (UBC12 homolog, yeast); UBC-RS2;
Gene ID	9040
mRNA Refseq	NM_003969
Protein Refseq	NP_003960
MIM	603173
UniProt ID	P61081
Chromosome Location	19q13.43
Pathway	Adaptive Immune System, organism-specific biosystem; Antigen processing: Ubiquitination & Proteasome degradation, organism-specific biosystem; Class I MHC mediated antigen processing & presentation, organism-specific biosystem; Immune System, organism-specific biosystem; Ubiquitin mediated proteolysis, organism-specific biosystem;
Function	ATP binding; NEDD8 ligase activity; acid-amino acid ligase activity; ligase activity; nucleotide binding; protein binding; ribosomal S6-glutamic acid ligase activity; ubiquitin-protein ligase activity; ubiquitin-protein ligase activity;

COA

Cat.No. :	UBE2M-6849H
Product Overview :	Recombinant human UBE2M protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography.