Recombinant Human UBE2M protein(Met1-Lys183)

• Cat.No.: UBE2M-857H
• Product Overview: Recombinant Human UBE2M (P61081) (Met 1-Lys 183) was expressed and purified in E. coli, with additional two amino acids (Gly & Pro) at the N-terminus.

Specification

• Source: E. coli
• Species: Human
• Tag: Non
• Protein length: Met1-Lys183
• Form: Lyophilized from sterile PBS, 10% glycerol, 1mM DTT, pH 8.0. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
• Molecular Mass: The recombinant human UBE2M consists of 185 amino acids and has a calculated molecular mass of 21 kDa. It migrates as an approximately 20 kDa band in SDS-PAGE under reducing conditions.
• Purity: > 90 % as determined by SDS-PAGE
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: UBE2M ubiquitin-conjugating enzyme E2M [ Homo sapiens ]
• Official Symbol: UBE2M
• Synonyms: UBE2M; ubiquitin-conjugating enzyme E2M; ubiquitin conjugating enzyme E2M (homologous to yeast UBC12) , ubiquitin conjugating enzyme E2M (UBC12 homolog, yeast); NEDD8-conjugating enzyme Ubc12; hUbc12; UBC12; yeast UBC12 homolog; NEDD8 protein ligase; NEDD8 carrier protein; ubiquitin-protein ligase M; ubiquitin carrier protein M; ubiquitin-conjugating enzyme E2M (UBC12 homolog, yeast); UBC-RS2
• Gene ID: 9040
• mRNA Refseq: NM_003969
• Protein Refseq: NP_003960
• MIM: 603173
• UniProt ID: P61081