Active Recombinant Full Length Human/Mouse/Rat ubiquitin conjugating enzyme E2 M Protein, Tag Free

• Cat.No.: UBE2M-10HFL
• Product Overview: Active Recombinant full length Human/Mouse/Rat ubiquitin conjugating enzyme E2 M Protein without tag was expressed in E. coli.

Specification

• Description: Ubiquitin-conjugating Enzyme E2M (UBE2M), also known as Ubiquitin-conjugating Enzyme 12 (Ubc12), is a ubiquitously expressed protein with a predicted molecular weight of 20 kDa. UBE2M/Ubc12 is highly conserved, and the human ortholog shares 100% amino acid identity with mouse and rat UBE2M. UBE2M/Ubc12 mediates conjugation of the Ubiquitin-like protein Neural Precursor Cell Expressed, Developmentally Down-regulated 8 (NEDD8) to substrate proteins. Neddylation requires the coordinated activities of UBE2M/Ubc12 and the APPBPI/UBA3 heterodimer, a NEDD8-activating (E1) enzyme. The NEDD8 pathway is essential for the viability of many organisms and plays important roles in morphogenesis and cell cycle progression. UBE2M/Ubc12-dependent neddylation modulates the levels of p27/Kip1, beta-Catenin, Cdc53/Cul1, and other members of the Cullin family.
• Source: E. coli
• Species: Human/Mouse/Rat
• Tag: Non
• Protein length: 1-183aa
• Bio-activity: Recombinant Human UBE2M/Ubc12 is a member of the NEDD8-conjugating (E2) enzyme family that receives NEDD8 from a NEDD8-
  activating (E1) enzyme and subsequently interacts with a NEDD8 ligase (E3) to conjugate NEDD8 to substrate proteins. Reaction conditions will need to be optimized for each specific application. We recommend an initial Recombinant Human UBE2M/Ubc12 concentration of 0.1-1 μM.
• Molecular Mass: 21 kDa
• Purity: > 95%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie Blue stain.
• Storage: Store the unopened product at -70 centigrade. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Do not use past expiration date.
• Storage Buffer: Supplied as a solution in HEPES, NaCl, TCEP and Glycerol.
• Shipping: The product is shipped with dry ice or equivalent.
• References: 1. Gong, L. et al. (1999) J. Biol. Chem. 274:12036.
  2. Huang, D.T. et al. (2005) Mol. Cell 17:341.
  3. Ohki, Y. et al. (2009) Biochem. Biophys. Res. Commun. 381:443.

Gene Information

• Gene Name: BE2M ubiquitin conjugating enzyme E2 M [ Homo sapiens (human) ]
• Official Symbol: UBE2M
• Synonyms: UBE2M; ubiquitin-conjugating enzyme E2M; ubiquitin conjugating enzyme E2M (homologous to yeast UBC12) , ubiquitin conjugating enzyme E2M (UBC12 homolog, yeast); NEDD8-conjugating enzyme Ubc12; hUbc12; UBC12; yeast UBC12 homolog; NEDD8 protein ligase; NEDD8 carrier protein; ubiquitin-protein ligase M; ubiquitin carrier protein M; ubiquitin-conjugating enzyme E2M (UBC12 homolog, yeast); UBC-RS2
• Gene ID: 9040
• mRNA Refseq: NM_003969
• Protein Refseq: NP_003960
• MIM: 603173
• UniProt ID: P61081