Recombinant Human Ubiquitin-Conjugating Enzyme E2, J2, His-tagged

• Cat.No.: UBE2J2-2485H
• Product Overview: Recombinant human UBE2J2 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Specification

• Description: UBE2J2 belongs to the ubiquitin-conjugating enzyme family. The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s.
• Source: E. coli
• Species: Human
• Tag: His
• Form: Liquid. In 20 mM Tris-HCl buffer, pH8.0, 30% glycerol, 1mM DTT, 200mM NaCl.
• Molecular Mass: 28.0kDa (250aa) confirmed by MALDI-TOF
• Protein length: 1-226aa
• AA Sequence: MGSSHHHHHH SSGLVPRGSH MGSHMSSTSS KRAPTTATQR LKQDYLRIKK DPVPYICAEP LPSNILEWHY VVRGPEMTPY EGGYYHGKLI FPREFPFKPP SIYMITPNGR FKCNTRLCLS ITDFHPDTWN PAWSVSTILT GLLSFMVEKG PTLGSIETSD FTKRQLAVQS LAFNLKDKVF CELFPEVVEE IKQKQKAQDE LSSRPQTLPL PDVVPDGETH LVQNGIQLLN GHAPGAVPNL AGLQQANRHH
• Purity: >90% by SDS - PAGE
• Applications: SDS-PAGE
• Storage: Can be stored at 4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C. Avoid repeated freezing and thawing cycles.
• Concentration: 1mg/ml (determined by Bradford assay)

Gene Information

• Gene Name: UBE2J2 ubiquitin-conjugating enzyme E2, J2 [ Homo sapiens ]
• Official Symbol: UBE2J2
• Synonyms: UBE2J2; ubiquitin-conjugating enzyme E2, J2; ubiquitin conjugating enzyme E2, J2 (UBC6 homolog, yeast); ubiquitin-conjugating enzyme E2 J2; NCUBE2; Ubc6p; yeast UBC6 homolog; ubiquitin conjugating enzyme 6; ubiquitin conjugating enzyme E2, J2; non-canonical ubiquitin-conjugating enzyme 2; ubiquitin-conjugating enzyme E2, J2 (UBC6 homolog, yeast); NCUBE-2; PRO2121
• Gene ID: 118424
• mRNA Refseq: NM_058167
• Protein Refseq: NP_477515
• UniProt ID: Q8N2K1
• Chromosome Location: 1p36.33
• Pathway: Adaptive Immune System, organism-specific biosystem; Antigen processing: Ubiquitination & Proteasome degradation, organism-specific biosystem; Class I MHC mediated antigen processing & presentation, organism-specific biosystem; Immune System, organism-specific biosystem; Parkinsons disease, organism-specific biosystem
• Function: ATP binding; acid-amino acid ligase activity; ligase activity; nucleotide binding; ubiquitin-protein ligase activity