Recombinant Human UBE2W Protein (A2-C151), His/Strep tagged

• Cat.No.: UBE2W-0047H
• Product Overview: Recombinant Human 8His-StrepII-TEV-(GG)-UBE2W(A2-C151 end) Protein was expressed in Insect cell.

Specification

• Description: Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. Specifically monoubiquitinates the N-terminus of various substrates, including ATXN3, MAPT/TAU, POLR2H/RPB8 and STUB1/CHIP, by recognizing backbone atoms of disordered N-termini. Involved in degradation of misfolded chaperone substrates by mediating monoubiquitination of STUB1/CHIP, leading to recruitment of ATXN3 to monoubiquitinated STUB1/CHIP, and restriction of the length of ubiquitin chain attached to STUB1/CHIP substrates by ATXN3. After UV irradiation, but not after mitomycin-C (MMC) treatment, acts as a specific E2 ubiquitin-conjugating enzyme for the Fanconi anemia complex by associating with E3 ubiquitin-protein ligase FANCL and catalyzing monoubiquitination of FANCD2, a key step in the DNA damage pathway. In vitro catalyzes 'Lys-11'-linked polyubiquitination. UBE2W-catalyzed ubiquitination occurs also in the presence of inactive RING/U-box type E3s, i.e. lacking the active site cysteine residues to form thioester bonds with ubiquitin, or even in the absence of E3, albeit at a slower rate.
• Source: Insect cell
• Species: Human
• Tag: His&Strep
• Form: Liquid
• Protein length: A2-C151
• Endotoxin: < 0.01 EU per μg of the protein
• Purity: 90%
• Stability: Samples are stable for up to twelve months from date of receipt at -20 to -80 centigrade.
• Storage: Store it under sterile conditions at -20 to -80 centigrade. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Storage Buffer: Supplied as sterile 50 mM Tris-HCl (pH7.5), 200 mM NaCl, 20% glycerol
• Shipping: It is shipped out with blue ice.

Gene Information

• Gene Name: UBE2W ubiquitin-conjugating enzyme E2W (putative) [ Homo sapiens (human) ]
• Official Symbol: UBE2W
• Synonyms: UBE2W; ubiquitin-conjugating enzyme E2W (putative); ubiquitin-conjugating enzyme E2 W; FLJ11011; ubiquitin-protein ligase W; ubiquitin carrier protein W; ubiquitin-conjugating enzyme 16; probable ubiquitin-conjugating enzyme E2 W; UBC16; UBC-16; hUBC-16
• Gene ID: 55284
• mRNA Refseq: NM_001001481
• Protein Refseq: NP_001001481
• MIM: 614277
• UniProt ID: Q96B02