Recombinant Human UBE2W, His-tagged

• Cat.No.: UBE2W-67H
• Product Overview: Recombinant humanUBE2W protein, fused to His-tag at N-terminus, was expressed in E. coli andpurified by using conventional chromatography techniques.

Specification

• Cat. No.: UBE2W-67H
• Description: UBE2Waccepts ubiquitin from the E1 complex and catalyzes its covalent attachmentto other proteins. This protein in vitro catalyzes monoubiquitination and"Lys-11"-linked polyubiquitination. UBE2W is widely expressed, especially athighest levels in testis.
• Form: Liquid. In 20mMTris-HCl buffer (pH 8.0) containing 10% glycerol, 2mM DTT, 0.1M NaCl.
• Molecular Weight: 19.5 kDa(171 aa), confirmed by MALDI-TOF.
• Purity: > 90%by SDS - PAGE
• Concentration: 1 mg/ml(determined by Bradford assay)
• Sequences of aminoacids: MGSSHHHHHHSSGLVPRGSH MASMQKRLQK ELLALQNDPP PGMTLNEKSV QNSITQWIVD MEGAPGTLYEGEKFQLLFKF SSRYPFDSPQ VMFTGENIPV HPHVYSNGHI CLSILTEDWS PALSVQSVCL SIISMLSSCKEKRRPPDNSF YVRTCNKNPK KTKWWYHDDT C
• Storage: Can bestored at +4°C short term (1-2 weeks). For long term storage, aliquot andstore at -20°C or -70°C. Avoid repeated freezing and thawing cycles.
• Protein length: 1-151 a.a.

Gene Information

• Gene Name: UBE2W ubiquitin-conjugatingenzyme E2W (putative) [ Homo sapiens ]
• Official Symbol: UBE2W
• Synonyms: UBE2W; ubiquitin-conjugating enzyme E2W(putative); UBC16; UBC-16; hUBC-16; FLJ11011; ubiquitin-conjugating enzyme E2W; ubiquitin-protein ligase W; ubiquitin carrier protein W;ubiquitin-conjugating enzyme 16; probable ubiquitin-conjugating enzyme E2 W;EC 6.3.2.19; OTTHUMP00000226882; OTTHUMP00000226883
• Gene ID: 55284
• mRNA Refseq: NM_018299
• Protein Refseq: NP_060769
• MIM: 614277
• UniProt ID: Q96B02
• Chromosome Location: 8q21.11
• Pathway: Adaptive Immune System; Antigen processing:Ubiquitination & Proteasome degradation; Class I MHC mediated antigenprocessing & presentation; Immune System; Ubiquitin mediated proteolysis
• Function: ATP binding; acid-amino acid ligaseactivity; ligase activity; nucleotide binding; ubiquitin-protein ligaseactivity