Recombinant Human UBE2D1, His-tagged

• Cat.No.: UBE2D1-66H
• Product Overview: Recombinant human UBE2D1 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography.

Specification

• Cat. No.: UBE2D1-66H
• Description: UBE2D1 belongs to the ubiquitin-conjugating enzyme family. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This gene encodes a member of the E2 ubiquitin-conjugating enzyme family. UBE2D1 is closely related to a stimulator of iron transport (SFT), and is up-regulated in hereditary hemochromatosis. It also functions in the ubiquitination of the tumor-suppressor protein p53 and the hypoxia-inducible transcription factor HIF1alpha by interacting with the E1 ubiquitin-activating enzyme and the E3 ubiquitin-protein ligases.
• Source: E. coli.
• Form: Liquid. 20mM Tris-HCl buffer (pH8.0) containing 40% glycerol, 0.1M NaCl
• Molecular weight: 19.0 kDa (170aa), confirmed by MALDI-TOF
• Purity: > 95% by SDS - PAGE
• Concentration: 1 mg/ml (determined by Bradford assay)
• Sequences of amino acids: MGSSHHHHHH SSGLVPRGSH MGSMALKRIQ KELSDLQRDP PAHCSAGPVG DDLFHWQATI MGPPDSAYQG GVFFLTVHFP TDYPFKPPKI AFTTKIYHPN INSNGSICLD ILRSQWSPAL TVSKVLLSIC SLLCDPNPDD PLVPDIAQIY KSDKEKYNRH AREWTQKYAM
• Storage: Can be stored at +4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C. Avoid repeated freezing and thawing cycles.

Gene Information

• Gene Name: UBE2D1 ubiquitin-conjugating enzyme E2D 1 (UBC4/5 homolog, yeast) [ Homo sapiens ]
• Synonyms: ubiquitin-conjugating enzyme E2D 1(UBC4/5 homolog, yeast); UBCH5; UBC4/5; UBCH5A; E2(17)KB1; UBC4/5 homolog; stimulator of Fe transport; UBE2D1; ubiquitin-protein ligase D1; ubiquitin carrier protein D1; SFT; ubiquitin-conjugating enzyme E2(17)KB 1; ubiquitin-conjugating enzyme E2-17 kDa 1; SFT; UbcH5A; EC 6.3.2.19; Ubiquitin-conjugating enzyme E2 D1; EC=6.3.2.19
• Gene ID: 7321
• mRNA Refseq: NM_003338
• Protein Refseq: NP_003329
• MIM: 602961
• UniProt ID: P51668
• Chromosome Location: 10q21.1
• Function: ATP binding; acid-amino acid ligase activity; ligase activity; protein binding; ubiquitin-protein ligase activity; nucleotide binding