Recombinant Human UBA1 Protein, MYC/DDK-tagged, C13 and N15-labeled

• Cat.No.: UBA1-264H
• Product Overview: UBA1 MS Standard C13 and N15-labeled recombinant protein (NP_003325) with a C-terminal MYC/DDK tag, was expressed in HEK293 cells.

Specification

• Description: The protein encoded by this gene catalyzes the first step in ubiquitin conjugation to mark cellular proteins for degradation. This gene complements an X-linked mouse temperature-sensitive defect in DNA synthesis, and thus may function in DNA repair. It is part of a gene cluster on chromosome Xp11.23. Alternatively spliced transcript variants that encode the same protein have been described. [provided by RefSeq, Jul 2008]
• Source: HEK293
• Species: Human
• Tag: Myc&DDK
• Molecular Mass: 117.8 kDa
• AA Sequence: MSSSPLSKKRRVSGPDPKPGSNCSP AQSVLSEVPSVPTNGMAKNGSEADI DEGLYSRQLYVLGHEAMKRLQTSSV LVSGLRGLGVEIAKNIILGGVKAVT LHDQGTAQWADLSSQFYLREEDIGK NRAEVSQPRLAELNSYVPVTAYTGP LVEDFLSGFQVVVLTNTPLEDQLRV GEFCHNRGIKLVVADTRGLFGQLFC DFGEEMILTDSNGEQPLSAMVSMVT KDNPGVVTCLDEARHGFESGDFVSF SEVQGMVELNGNQPMEIKVLGPYTF SICDTSNFSDYIRGGIVSQVKVPKK ISFKSLVASLAEPDFVVTDFAKFSR PAQLHIGFQALHQFCAQHGRPPRPR NEEDAAELVALAQAVNARALPAVQQ NNLDEDLIRKLAYVAAGDLAPINAF IGGLAAQEVMKACSGKFMPIMQWLY FDALECLPEDKEVLTEDKCLQRQNR YDGQVAVFGSDLQEKLGKQKYFLVG AGAIGCELLKNFAMIGLGCGEGGEI IVTDMDTIEKSNLNRQFLFRPWDVT KLKSDTAAAAVRQMNPHIRVTSHQN RVGPDTERIYDDDFFQNLDGVANAL DNVDARMYMDRRCVYYRKPLLESGT LGTKGNVQVVIPFLTESYSSSQDPP EKSIPICTLKNFPNAIEHTLQWARD EFEGLFKQPAENVNQYLTDPKFVER TLRLAGTQPLEVLEAVQRSLVLQRP QTWADCVTWACHHWHTQYSNNIRQL LHNFPPDQLTSSGAPFWSGPKRCPH PLTFDVNNPLHLDYVMAAANLFAQT YGLTGSQDRAAVATFLQSVQVPEFT PKSGVKIHVSDQELQSANASVDDSR LEELKATLPSPDKLPGFKMYPIDFE KDDDSNFHMDFIVAASNLRAENYDI PSADRHKSKLIAGKIIPAIATTTAA VVGLVCLELYKVVQGHRQLDSYKNG FLNLALPFFGFSEPLAAPRHQYYNQ EWTLWDRFEVQGLQPNGEEMTLKQF LDYFKTEHKLEITMLSQGVSMLYSF FMPAAKLKERLDQPMTEIVSRVSKR KLGRHVRALVLELCCNDESGEDVEV PYVRYTIRTRTRPLEQKLISEEDLA ANDILDYKDDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Stability: Stable for 3 months from receipt of products under proper storage and handling conditions.
• Storage: Store at -80 centigrade. Avoid repeated freeze-thaw cycles.
• Concentration: 50 μg/mL as determined by BCA
• Storage Buffer: 100 mM glycine, 25 mM Tris-HCl, pH 7.3.

Gene Information

• Gene Name: UBA1 ubiquitin-like modifier activating enzyme 1 [ Homo sapiens (human) ]
• Official Symbol: UBA1
• Synonyms: UBA1; ubiquitin-like modifier activating enzyme 1; A1S9T, GXP1, UBE1, ubiquitin activating enzyme E1, ubiquitin activating enzyme E1 (A1S9T and BN75 temperature sensitivity complementing); ubiquitin-like modifier-activating enzyme 1; POC20; POC20 centriolar protein homolog (Chlamydomonas); ubiquitin activating enzyme E1 homolog (yeast); UBE1X; POC20 centriolar protein homolog; UBA1, ubiquitin-activating enzyme E1 homolog A; A1S9T and BN75 temperature sensitivity complementing; A1S9; A1ST; GXP1; UBE1; A1S9T; AMCX1; SMAX2; UBA1A; MGC4781
• Gene ID: 7317
• mRNA Refseq: NM_003334
• Protein Refseq: NP_003325
• MIM: 314370
• UniProt ID: P22314

Reviews

07/02/2022

The UBA1 protein comes highly recommended for its outstanding performance in various experimental applications.

07/07/2019

In ELISA assays, it has consistently exhibited exceptional reliability and accuracy, providing precise and reproducible results.

05/13/2017

Their prompt and knowledgeable guidance has been instrumental in effectively addressing my queries and optimizing the utilization of the UBA1 protein in my studies.

Q&As

Are there any clinical trials involving UBA1 as a target?

Some early-phase clinical trials are exploring UBA1 as a potential target in the context of ALS and cancer.

How can UBA1 be targeted for therapeutic purposes?

Targeting UBA1 with small molecules or gene therapy may provide a potential avenue for treating UBA1-related diseases.

How does UBA1 function in the cell?

UBA1 activates ubiquitin, a small protein that tags other proteins for degradation by the proteasome.

What diseases are associated with UBA1 dysfunction?

UBA1 dysfunction has been linked to neurodegenerative diseases like amyotrophic lateral sclerosis (ALS) and certain types of cancer.

Can UBA1 be a diagnostic marker for ALS?

Research suggests that altered UBA1 levels in the cerebrospinal fluid may be a potential biomarker for ALS diagnosis.