Recombinant Human TYRP1 protein, His-tagged

• Cat.No.: TYRP1-22H
• Product Overview: Recombinant human TYRP1, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.

Specification

• Species: Human
• Source: Insect Cells
• Tag: His
• ProteinLength: 25-477 a.a.
• Description: TYRP1, also known as 5, 6-dihydroxyindole-2-carboxylic acid oxidase, is a melanosomal enzyme that belongs to the tyrosinase family and plays an important role in the melanin biosynthetic pathway. It is a melanocyte-specific gene product involved in eumelanin synthesis. It is involved in the oxidation of 5,6-dihydroxyindole-2-carboxylic acid(DHICA) into indole-5,6-quinone-2-carboxylic acid. It is regulated by the microphthalmia-associated transcription factor(MITF).
• Form: Liquid. In Phosphate Buffered Saline (pH 7.4) containing 10% glycerol
• Molecular Mass: 52.5kDa (462aa)
  50-70kDa (SDS-PAGE under reducing conditions)
• AA Sequence: ADLQFPRQCA TVEALRSGMC CPDLSPVSGP GTDRCGSSSG RGRCEAVTAD SRPHSPQYPH DGRDDREVWP LRFFNRTCHC NGNFSGHNCGTCRPGWRGAA CDQRVLIVRR NLLDLSKEEK NHFVRALDMA KRTTHPLFVI ATRRSEEILG PDGNTPQFEN ISIYNYFVWT HYYSVKKTFLGVGQESFGEV DFSHEGPAFL TWHRYHLLRL EKDMQEMLQE PSFSLPYWNF ATGKNVCDIC TDDLMGSRSN FDSTLISPNS VFSQWRVVCDSLEDYDTLGT LCNSTEDGPI RRNPAGNVAR PMVQRLPEPQ DVAQCLEVGL FDTPPFYSNS TNSFRNTVEG YSDPTGKYDP AVRSLHNLAH
  LFLNGTGGQT HLSPNDPIFV LLHTFTDAVF DEWLRRYNAD ISTFPLENAP IGHNRQYNMV PFWPPVTNTE MFVTAPDNLG YTYEIQWPSREFSVPEHHHH HH
• Endotoxin: < 1.0 EU per 1 microgram of protein (determined by LAL method)
• Purity: > 85% by SDS - PAGE
• Storage: Can be stored at +4centigrade short term (1-2 weeks). For long term storage, aliquot and store at -20centigrade or -70centigrade. Avoid repeated freezing and thawing cycles.
• Concentration: 0.25mg/ml (determined by Absorbance at 280nm)

Gene Information

• Gene Name: TYRP1 tyrosinase-related protein 1 [ Homo sapiens ]
• Official Symbol: TYRP1
• Synonyms: TYRP1; tyrosinase-related protein 1; CAS2, TYRP; CATB; GP75; TRP; TRP1; TRP-1; catalase B; DHICA oxidase; glycoprotein 75; melanoma antigen gp75; CAS2; TYRP; b-PROTEIN
• Gene ID: 7306
• mRNA Refseq: NM_000550
• Protein Refseq: NP_000541
• MIM: 115501
• UniProt ID: P17643
• Chromosome Location: 9p23
• Pathway: Direct p53 effectors, organism-specific biosystem; Melanogenesis, organism-specific biosystem; Melanogenesis, conserved biosystem; Metabolic pathways, organism-specific biosystem; Tyrosine metabolism, organism-specific biosystem; Tyrosine metabolism, conserved biosystem; ethanol degradation IV, organism-specific biosystem
• Function: copper ion binding; metal ion binding; oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, another compound as one donor, and incorporation of one atom of oxygen; protein binding; protein heterodimerization activity; protein homodimerization activity