Recombinant Human TWF1, His-tagged

• Cat.No.: TWF1-30334TH
• Product Overview: Recombinant fragment Human PTK9 with an N terminal His tag; 277 aa, 31.5 kDa incluive of tag.

Specification

• Species: Human
• Source: E.coli
• Tag: His
• ProteinLength: 251 amino acids
• Description: This gene encodes twinfilin, an actin monomer-binding protein conserved from yeast to mammals. Studies of the mouse counterpart suggest that this protein may be an actin monomer-binding protein, and its localization to cortical G-actin-rich structures may be regulated by the small GTPase RAC1.
• Conjugation: HIS
• Molecular Weight: 31.500kDa inclusive of tags
• Tissue specificity: Expressed at high levels in the colon, testis, ovary, prostate and lung. Expressed at lower levels in the brain, bladder and heart. Not detected in liver.
• Form: Liquid
• Purity: by SDS-PAGE
• Storage buffer: pH: 8.00Constituents:0.32% Tris HCl, 40% Glycerol, 0.88% Sodium chloride, 0.02% DTT
• Storage: Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
• Sequences of amino acids: MGSSHHHHHH SSGLVPRGSH MGSHM MLYAA TRATLKKEFG GGHIKDEVFG TVKEDVSLHG YKKYLLSQSS PAPLTAAEEE LRQIKINEVQ TDVGVDTKHQ TLQGVAFPIS REAFQALEKL NNRQLNYVQL EIDIKNEIII LANTTNTELK DLPKRIPKDS ARYHFFLYKH SHEGDYLESI VFIYSMPGYT CSIRERMLYS SCKSRLLEIV ERQLQMDVIR KIEIDNGDEL TADFLYEEVH PKQHAHKQSF AKPKGPAGKR GIRRLIRGPA ETEATTD
• Sequence Similarities: Belongs to the actin-binding proteins ADF family. Twinfilin subfamily.Contains 2 ADF-H domains.

Gene Information

• Gene Name: TWF1 twinfilin, actin-binding protein, homolog 1 (Drosophila) [ Homo sapiens ]
• Official Symbol: TWF1
• Synonyms: TWF1; twinfilin, actin-binding protein, homolog 1 (Drosophila); protein tyrosine kinase 9 , PTK9, PTK9 protein tyrosine kinase 9; twinfilin-1; A6
• Gene ID: 5756
• mRNA Refseq: NM_001242397
• Protein Refseq: NP_001229326
• MIM: 610932
• Uniprot ID: Q12792
• Chromosome Location: 12q12
• Pathway: superpathway of gluconate degradation, organism-specific biosystem
• Function: ATP binding; actin monomer binding; phosphatidylinositol-4,5-bisphosphate binding; protein tyrosine kinase activity; NOT protein tyrosine kinase activity