Recombinant Human Twinfilin, Actin-Binding Protein, Homolog 1 (Drosophila), His-tagged

• Cat.No.: TWF1-1322H
• Product Overview: Recombinant human TWF1 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography.

Specification

• Species: Human
• Source: E.coli
• Tag: His
• Description: TWF1 is a ubiquitous actin-monomer-binding protein that is composed of two ADF-homology domains. It forms a 1:1 complex with ADP-actin-monomers, inhibits nucleotide exchange on actin monomers and prevents assembly of the monomer into filaments.
• Form: Liquid. In 20 mM Tris-HCl buffer (pH 8.0) containing 1 mM DTT, 40% glycerol, 0.15 M NaCl.
• Molecular Weight: 31.5 kDa (277 aa) confirmed by MALDI-TOF
• Purity: > 80 % by SDS-PAGE
• Concentration: 0.25 mg/ml (determined by Bradford assay)
• Sequences of amino acids: MGSSHHHHHH SSGLVPRGSH MGSHMMLYAA TRATLKKEFG GGHIKDEVFG TVKEDVSLHG YKKYLLSQSS PAPLTAAEEE LRQIKINEVQ TDVGVDTKHQ TLQGVAFPIS REAFQALEKL NNRQLNYVQL EIDIKNEIII LANTTNTELK DLPKRIPKDS ARYHFFLYKH SHEGDYLESI VFIYSMPGYT CSIRERMLYS SCKSRLLEIV ERQLQMDVIR KIEIDNGDEL TADFLYEEVH PKQHAHKQSF AKPKGPAGKR GIRRLIRGPA ETEATTD
• Storage: Can be stored at +4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C. Avoid repeated freezing and thawing cycles.
• Pathways: superpathway of gluconate degradation

Gene Information

• Gene Name: TWF1 twinfilin, actin-binding protein, homolog 1 (Drosophila) [ Homo sapiens ]
• Official Symbol: TWF1
• Synonyms: TWF1; twinfilin, actin-binding protein, homolog 1 (Drosophila); A6; PTK9; MGC23788; MGC41876; twinfilin-1; protein A6; protein tyrosine kinase 9; A6 protein tyrosine kinase; PTK9 protein tyrosine kinase 9
• Gene ID: 5756
• mRNA Refseq: NM_001242397
• Protein Refseq: NP_001229326
• MIM: 610932
• UniProt ID: Q12792
• Chromosome Location: 12q12
• Function: ATP binding; actin monomer binding; phosphatidylinositol-4,5-bisphosphate binding; protein tyrosine kinase activity; NOT protein tyrosine kinase activity