Recombinant Human TSEN2 Protein, Myc/DDK-tagged, C13 and N15-labeled

• Cat.No.: TSEN2-3157H
• Product Overview: TSEN2 MS Standard C13 and N15-labeled recombinant protein (NP_001138864) with a C-terminal MYC/DDK tag, was expressed in HEK293 cells.

Specification

• Description: This gene encodes one of the subunits of the tRNA splicing endonuclease. This endonuclease catalyzes the first step in RNA splicing which is the removal of introns. Mutations in this gene have been associated with pontocerebellar hypoplasia type 2. A pseudogene has been identified on chromosome 4. Multiple transcript variants encoding different isoforms have been found for this gene.
• Source: HEK293
• Species: Human
• Tag: Myc&DDK
• Molecular Mass: 53.2 kDa
• AA Sequence: MAEAVFHAPKRKRRVYETYESPLPIPFGQDHGPLKEFKIFRAEMINNNVIVRNAEDIEQLYGKGYFGKGILSRSRPSFTISDPKLVAKWKDMKTNMPIITSKRYQHSVEWAAELMRRQGQDESTVRRILKDYTKPLEHPPVKRNEEAQVHDKLNSGMVSNMEGTAGGERPSVVNGDSGKSGGVGDPREPLGCLQEGSGCHPTTESFEKSVREDASPLPHVCCCKQDALILQRGLHHEDGSQHIGLLHPGDRGPDHEYVLVEEAECAMSEREAAPNEELVQRNRLICRRNPYRIFEYLQLSLEEAFFLVYALGCLSIYYEKEPLTIVKLWKAFTVVQPTFRTTYMAYHYFRSKGWVPKVGLKYGTDLLLYRKGPPFYHASYSVIIELVDDHFEGSLRRPLSWKSLAALSRVSVNVSKELMLCYLIKPSTMTDKEMESPECMKRIKVQEVILSRWVSSRERSDQDDLTRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Stability: Stable for 3 months from receipt of products under proper storage and handling conditions.
• Storage: Store at -80 centigrade. Avoid repeated freeze-thaw cycles.
• Concentration: 50 μg/mL as determined by BCA
• Storage Buffer: 100 mM glycine, 25 mM Tris-HCl, pH 7.3.

Gene Information

• Gene Name: TSEN2 tRNA splicing endonuclease subunit 2 [ Homo sapiens (human) ]
• Official Symbol: TSEN2
• Synonyms: TSEN2; tRNA splicing endonuclease 2 homolog (S. cerevisiae); tRNA splicing endonuclease 2 homolog (SEN2, S. cerevisiae); tRNA-splicing endonuclease subunit Sen2; MGC2776; SEN2; SEN2L; hsSen2; tRNA-intron endonuclease Sen2; PCH2B; MGC4440
• Gene ID: 80746
• mRNA Refseq: NM_001145392
• Protein Refseq: NP_001138864
• MIM: 608753
• UniProt ID: Q8NCE0