Recombinant Human TSEN15 tRNA splicing endonuclease subunit, His-tagged

• Cat.No.: TSEN15-693H
• Product Overview: TSEN15, 1-171aa, Human, His tag, E.coli

Specification

• Description: TSEN15 (tRNA-splicing endonuclease subunit Sen15) belongs to the SEN15 family. tRNA splicing is a fundamental process required for cell growth and division. SEN15 is a subunit of the tRNA splicing endonuclease. It cleaves pre-tRNA at the 5" and 3" splice sites to release the intron. Recombinant human TSEN15 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.
• Source: E.coli
• Species: Human
• Tag: His-tag
• Form: Liquid. In 20mM Tris-HCl buffer (pH 8.0) containing 2mM DTT, 20% glycerol, 200mM NaCl
• Molecular Mass: 20.9kDa (191aa) confirmed by MALDI-TOF
• AA Sequence: MGSSHHHHHH SSGLVPRGSH MEERGDSEPT PGCSGLGPDG VRGFGDGGGA PSWAPEDAWM GTHPKYLEMM ELDIGDATHV YVAFLVYLDL MESKSWHEVN CVGLPELQLI CLVGTEIEGE GLQTVVPTPI TASLSHNRIR EILKASRKLQ GDPDLPMSFT LAIVESDSTI VYYKLTDGFM LPDPQNISLR R
• Purity: >90% by SDS - PAGE
• Storage: Can be stored at +4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C. Avoid repeated freezing and thawing cycles.
• Concentration: 0.5mg/ml (determined by Bradford assay)
• Protein length: 1-171 a.a.

Gene Information

• Gene Name: TSEN15 tRNA splicing endonuclease 15 homolog (S. cerevisiae) [ Homo sapiens ]
• Official Symbol: TSEN15
• Synonyms: TSEN15; tRNA splicing endonuclease 15 homolog (S. cerevisiae); C1orf19, chromosome 1 open reading frame 19; tRNA-splicing endonuclease subunit Sen15; hsSen15; SEN15 homolog; tRNA-intron endonuclease Sen15; sen15; C1orf19
• Gene ID: 116461
• mRNA Refseq: NM_001127394
• Protein Refseq: NP_001120866
• MIM: 608756
• UniProt ID: Q8WW01
• Chromosome Location: 1q25