Recombinant Human Transcription Elongation Factor B (SIII), Polypeptide 1 (15kDa, elongin C), His-tagged

• Cat.No.: TCEB1-4832H
• Product Overview: Recombinant human TCEB1 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

Specification

• Species: Human
• Source: E.coli
• Tag: His
• Description: TCEB1 (elongin C) is a subunit of elongin, which is a general transcription elongation factor that increases the RNA polymerase II transcription elongation past template encoded arresting sites. The SIII complex is composed of elongins A/A2, B and C. It activates elongation by RNA polymerase II by suppressing transient pausing of the polymerase at many sites within transcription units. Elongin A functions as the transcriptionally active component of the SIII complex, whereas elongins B and C are regulatory subunits. Elongin A2 is specifically expressed in the testis, and capable of forming a stable complex with elongins B and C. The von Hippel-Lindau(VHL) tumor suppressor protein binds to elongins B and C, and thereby inhibits transcription elongation.
• Form: Liquid. In 20mM Tris-HCl buffer (pH 8.0) containing10% glycerol, 1mM DTT, 0.15M NaCl.
• Molecular Weight: 14.6 kDa (132aa), confirmed by MALDI-TOF.
• Purity: >90% by SDS-PAGE
• Concentration: 1 mg/ml (determined by Bradford)
• Sequences of amino acids: MGSSHHHHHH SSGLVPRGSH MDGEEKTYGG CEGPDAMYVK LISSDGHEFI VKREHALTSG TIKAMLSGPG QFAENETNEV NFREIPSHVL SKVCMYFTYK VRYTNSSTEI PEFPIAPEIA LELLMAANFL DC
• Storage: Can be stored at +4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C. Avoid repeated freezing and thawing cycles.
• Pathways: Adaptive Immunity Signaling; Antigen processing: Ubiquitination& Proteasome degradation; Class I MHC mediated antigen processing & presentation; ECS complex; ECV complex; Formation and Maturation of mRNA Transcript; Formation of HIV-1 elongation complex containing HIV-1 Tat; Formation of HIV-1 elongation complex in the absence of HIV-1 Tat; Formation of RNA Pol II elongation complex; Gene Expression; HIF-2-alpha transcription factor network; HIV Infection; HIV Life Cycle; HIV-1 Transcription Elongation; HIV-1 elongation arrest and recovery

Gene Information

• Gene Name: TCEB1 transcription elongation factor B (SIII), polypeptide 1 (15kDa, elongin C) [ Homo sapiens ]
• Official Symbol: TCEB1
• Synonyms: TCEB1; transcription elongation factor B (SIII), polypeptide 1 (15kDa, elongin C); SIII; transcription elongation factor B polypeptide 1; eloC; SIII p15; elongin-C; OTTHUMP00000226846; OTTHUMP00000226847; OTTHUMP00000226848; OTTHUMP00000226849; OTTHUMP00000226850; OTTHUMP00000226851; OTTHUMP00000226852; OTTHUMP00000227658; elongin 15 kDa subunit; transcription elongation factor B, polypeptide 1; RNA polymerase II transcription factor SIII subunit C; Elongin 15 kDa subunit; transcription elongation factor B polypeptide 1; EloC; transcription elongation factor B (SIII), polypeptide 1 (15kD, elongin C); Elongin-C
• Gene ID: 6921
• mRNA Refseq: NM_001204857
• Protein Refseq: NP_001191786
• MIM: 600788
• UniProt ID: Q15369
• Chromosome Location: 8q21.11
• Function: protein binding