Recombinant Human TNFSF8, FLAG-tagged

• Cat.No.: TNFSF8-2076H
• Product Overview: Recombinant Human TNFSF8 is produced inHEK 293 Cells. The extracellular domain of recombinant human CD30L (CD153) (aa 63-234) is fused at the N-terminus to a linker peptide (11 aa) and a FLAG-tag. MW=35kDa.

Specification

• Species: Human
• Source: HEK293
• Tag: Flag
• Protein Length: 63-234 a.a.
• Description: The protein encoded by this gene is a cytokine that belongs to the tumor necrosis factor (TNF) ligand family. This cytokine is a ligand for TNFRSF8/CD30, which is a cell surface antigen and a marker for Hodgkin lymphoma and related hematologic malignancies. The engagement of this cytokine expressed on B cell surface plays an inhibitory role in modulating Ig class switch. This cytokine was shown to enhance cell proliferation of some lymphoma cell lines, while to induce cell death and reduce cell proliferation of other lymphoma cell lines.
• Purity: ≥95% (SDS-PAGE).
• Concentration: 0.1mg/ml after reconstitution.
• Formulation: Lyophilized. Contains PBS.
• Endotoxin Content: <0.1EU/µg purified protein (LAL test; Bio Whittaker).
• Reconstitution: Reconstitute with 100µl sterile distilled water. Further dilutions should be made with medium containing 5% fetal calf serum.
• Specificity: Binds to human CD30 (Ki-1).
• Storage: Stable for at least 6 months after receipt when stored at -20°C.
• Handling: After reconstitution, prepare aliquots and store at -20°C. Avoid freeze/thaw cycles.

Gene Information

• Gene Name: TNFSF8 tumor necrosis factor (ligand) superfamily, member 8 [ Homo sapiens ]
• Synonyms: TNFSF8; tumor necrosis factor (ligand) superfamily, member 8; CD153; CD30-L; CD30L; CD30LG; MGC138144; CD153 antigen; CD30 antigen ligand; CD30 ligand; OTTHUMP00000022762
• Gene ID: 944
• mRNA Refseq: NM_001244
• Protein Refseq: NP_001235
• MIM: 603875
• UniProt ID: P32971
• Chromosome Location: 9q33
• Pathway: kine-cytokine receptor interaction
• Function: cytokine activity; tumor necrosis factor receptor binding