Active Recombinant Cynomolgus TNFSF8, Fc tagged

• Cat.No.: TNFSF8-8821C
• Product Overview: Recombinant Cynomolgus TNFSF8 (G7P2F1) (Gln63-Asp234), fused with the Fc region of human IgG1 at the N-terminus, was produced in Human Cells.

Specification

• Source: HEK293
• Species: Cynomolgus
• Tag: Fc
• Form: Lyophilized from sterile 50 mM Tris, pH 8.0.
  Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
• Bio-activity: Immobilized Cynomolgus Fc-TNFSF8 at 10 μg/ml (100 μl/well) can bind biotinylated human CD30-Fch, The EC50 of biotinylated human CD30-Fch is 5.3-12.5 ng/ml.
• Molecular Mass: The recombinant cynomolgus TNFSF8 is a disulfide-linked homodimer. The reduced monomer comprises 432 amino acids and has a calculated molecular mass of 48.1 KDa.The apparent molecular mass of the protein is approximately 59 KDa respectively in SDS-PAGE.
• Protein length: Gln63-Asp234
• Endotoxin: < 1.0 EU per μg protein as determined by the LAL method.
• Purity: > 90 % as determined by SDS-PAGE.
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C
  Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4℃ before opening to recover the entire contents.