Recombinant Human TNFSF8 protein, His-tagged, low endotoxin

• Cat.No.: TNFSF8-829H
• Product Overview: Recombinant Human TNFSF8 (Gln63-Asp234) protein (low endotoxin) was fused to His-tag at N-terminus and expressed in human 293 cells (HEK293).

Specification

• Description: CD30 ligand (CD30L) and CD30 are interacting cellsurface glycoproteins that are members of the tumour necrosis factor (TNF) and tumour necrosis factor receptor (TNFR) superfamilies. In humans, CD30L is expressed at high levels on activated T cells. There is conflicting evidence with regard to cell surface expression of CD30L on primary human B cells. An initial report of constitutive CD30L expression on peripheral blood B cells33 has not been substantiated.
• Source: HEK293
• Species: Human
• Tag: His
• Predicted N Terminal: Gln63
• Form: Lyophilized from 0.22 μm filtered solution in PBS, pH7.4, 10% trehalose.
• Molecular Mass: The protein has a calculated MW of 21.5 kDa. The protein migrates as 30-40 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
• Protein length: Gln63-Asp234
• Endotoxin: Less than 0.01 EU per μg by the LAL method.
• Purity: >90% as determined by SDS-PAGE.
• Storage: For long term storage, the product should be stored at lyophilized state at -20 centigrade or lower.
  Please avoid repeated freeze-thaw cycles.
  This product is stable after storage at:
  -20 to -70 centigrade for 12 months in lyophilized state;
  -70 centigrade for 3 months under sterile conditions after reconstitution.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4℃ before opening to recover the entire contents.

Gene Information

• Gene Name: TNFSF8
• Official Symbol: TNFSF8
• Synonyms: CD153, CD30L, CD30LG, TNLG3A
• Gene ID: 944
• mRNA Refseq: NM_001244.3
• Protein Refseq: NP_001235.1
• MIM: 603875
• UniProt ID: P32971