Recombinant Human TNFSF11, His-tagged

• Cat.No.: TNFSF11-3553H
• Product Overview: Recombinant humanTNFSF11 protein, fused to His-tag at N-terminus, was expressed in E. coli andpurified by using conventional chromatography.

Specification

• Cat. No.: TNFSF11-3553H
• Description: TNFSF11,also known as RANKL, is a member of the tumor necrosis factor (TNF) cytokinefamily which is a ligand for osteoprotegerin and functions as a key factorfor osteoclast differentiation and activation. TNFSF11 also has a function inthe immune system, where it is expressed by T helper cells and is thought tobe involved in dendritic cell maturation. TNFSF11 is important in bonemetabolism. This natural and necessary surface- bound molecule (also known asCD254) found on osteoblasts serves to activate osteoclasts, which are the cellsinvolved in bone resorption.
• Form: Liquid. 20mM Tris-HClbuffer (pH8.0) containing 20% glycerol, 0.1M NaCl, 1mM DTT.
• Molecular Weight: 22.3 kDa(199 aa), confirmed by MALDI-TOF.
• Purity: > 80%by SDS - PAGE
• Concentration: 0.5 mg/ml(determined by Bradford assay)
• Sequences of aminoacids: MGSSHHHHHHSSGLVPRGSH M IRAEKAMVD GSWLDLAKRS KLEAQPFAHL T INATDIPSG SHKVSLSSWYHDRGWAKISN MTFSNGKLIV NQDGFYYLYA NICFRHHETS GDLATEYLQL MVYVTKTSIK IPSSHTLMKGGSTKYWSGNS EF HFYSINVG GFFKLRSGEE ISIEVSNPSL LDPDQDATYF GAFKVRDID
• Storage: Can bestored at +4°C short term (1-2 weeks). For long term storage, aliquot andstore at -20°C or -70°C. Avoid repeated freezing and thawing cycles.

Gene Information

• Gene Name: TNFSF11 tumor necrosis factor(ligand) superfamily, member 11 [ Homo sapiens ]
• Official Symbol: TNFSF11
• Synonyms: TNFSF11; tumor necrosis factor (ligand)superfamily, member 11; ODF; OPGL; sOdf; CD254; OPTB2; RANKL; TRANCE;hRANKL2; tumor necrosis factor ligand superfamily member 11; osteoprotegerinligand; osteoclast differentiation factor; TNF-related activation-inducedcytokine; receptor activator of nuclear factor kappa B ligand; receptoractivator of nuclear factor kappa-B ligand; OTTHUMP00000018328;OTTHUMP00000178585
• Gene ID: 8600
• mRNA Refseq: NM_003701
• Protein Refseq: NP_003692
• MIM: 602642
• UniProt ID: O14788
• Chromosome Location: 13q14
• Pathway: Cytokine-cytokine receptor interaction;IL6-mediated signaling events; Osteoblast Signaling; Osteoclastdifferentiation; Rheumatoid arthritis
• Function: cytokine activity; receptor activity; tumornecrosis factor receptor binding; tumor necrosis factor receptor superfamilybinding