Active Recombinant Human TNFSF11 Protein, His-tagged

• Cat.No.: TNFSF11-873H
• Product Overview: Recombinant Human TNFSF11(Gly136-Asp317), transcript variant 1, fused with His tag at N-terminal was expressed in HEK293 cells.

Specification

• Description: This gene encodes a member of the tumor necrosis factor (TNF) cytokine family which is a ligand for osteoprotegerin and functions as a key factor for osteoclast differentiation and activation. This protein was shown to be a dentritic cell survival factor and is involved in the regulation of T cell-dependent immune response. T cell activation was reported to induce expression of this gene and lead to an increase of osteoclastogenesis and bone loss. This protein was shown to activate antiapoptotic kinase AKT/PKB through a signaling complex involving SRC kinase and tumor necrosis factor receptor-associated factor (TRAF) 6, which indicated this protein may have a role in the regulation of cell apoptosis. Targeted disruption of the related gene in mice led to severe osteopetrosis and a lack of osteoclasts. The deficient mice exhibited defects in early differentiation of T and B lymphocytes, and failed to form lobulo-alveolar mammary structures during pregnancy. Two alternatively spliced transcript variants have been found.
• Source: HEK293
• Tag: His
• Form: 1 x PBS, pH6.5
• Bio-activity: Bioactivity was measured by its property to induce osteoclast differentiation in RAW264.7 cells in the presence of 2.5 µg/ml of anti-His tag antibody (Cat. No. 652501).
• Molecular Mass: 22.7 kDa
• Purity: >95%, as determined by Coomassie stained SDS-PAGE.
• Concentration: Protein formulated and vialed at >50 ug/mL in specified buffer
• Protein length: 136-317 a.a.

Gene Information

• Gene Name: TNFSF11 tumor necrosis factor (ligand) superfamily, member 11 [ Homo sapiens ]
• Official Symbol: TNFSF11
• Synonyms: TNFSF11; tumor necrosis factor (ligand) superfamily, member 11; tumor necrosis factor ligand superfamily member 11; CD254; ODF; OPGL; RANKL; TRANCE; osteoprotegerin ligand; osteoclast differentiation factor; TNF-related activation-induced cytokine; receptor activator of nuclear factor kappa B ligand; receptor activator of nuclear factor kappa-B ligand; sOdf; OPTB2; hRANKL2
• Gene ID: 8600
• mRNA Refseq: NM_003701
• Protein Refseq: NP_003692
• MIM: 602642
• UniProt ID: O14788