Recombinant Human TNFRSF4, Fc Chimera

• Cat.No.: TNFRSF4-455H
• Product Overview: Recombinant Human Ox40 was fused to the Fc region of human IgG1 (93-330). The chimeric protein was expressed in modifiedhuman 293 cells.

Specification

• Species: Human
• Source: HEK293
• Tag: Fc
• Protein Length: 93-330 a.a.
• Description: Ox40 is a member of the tumor necrosis factor receptor (TNFR) family. It is a type I transmembrane protein expressed primarily on the surface of activated CD4+ T-cells. It is also expressed at low levels on the surface of activated CD8+ T cells, B cells, dendritic cells and eosinophils. Ox40 is a co-stimulatory molecule involved in T-cell activation and proliferation, the induction of cytokine production by effector T-cells, generation of memory T-cells, and arresting peripheral T-cell tolerance in vivo. Expression of Ox40 is induced following the initiation of a CD28 signal. It has been reported that the interaction of Ox40 with its ligand plays a role in the expansion of T-cell numbers at the height of the immune response as well as the generation of memory T-cells. Human Ox40 comprises a 186 amino acid extracellular domain containing four TNFR-Cys repeats, followed by a 21 amino acid transmembrane domain and a 42 amino acid cytoplasmic domain. Ox40 activity is mediated by NF-kappaB signaling via interaction of the intracellular domain of Ox40 with TRAF proteins.
• Molecular Mass: Ox40-Fc Chimera igrates as a broad band between 55 and 65 kDa in SDS-PAGE due to post-translation modifications, in particular glycosylation. This compares with the unmodified Ox40-Fc Chimera that has a predicted molecular mass of 46.4kDa.
• PI: Ox40-Fc Chimera separates into a number of isoforms with a pI between 5.5 and 7.9 in 2D PAGE due to post-translational modifications, in particular glycosylation. This compares with the unmodified Ox40-Fc Chimera that has a predicted pI of 8.32.
• % Carbohydrate: purified Ox40-Fc Chimera consists of 15-30% carbohydrate by weight.
• Glycosylation: Ox40-Fc Chimera has N-linked and O-linked oligosaccharides.
• Purity: >95%, as determined by SDS-PAGE and visualized by silver stain.
• Formulation: When reconstituted in 0.5 ml sterile phosphate-buffered saline, the solution will contain 1% human serum albumin (HSA) and 10% trehalose.
• Reconstitution: It is recommended that 0.5 ml of sterile phosphate-buffered saline be added to the vial.
• Storage: Lyophilized products should be stored at 2 to 8°C. Following reconstitution short-term storage at 4°C is recommended, and longer-term storage of aliquots at -18 to -20°C.

Gene Information

• Gene Name: TNFRSF4 tumor necrosis factor receptor superfamily, member 4 [ Homo sapiens ]
• Synonyms: tumor necrosis factor receptor superfamily, member 4; OX40; ACT35; CD134; TXGP1L; TNFRSF4; tax-transcriptionally activated glycoprotein 1 receptor; lymphoid activation antigene ACT35; OX40 cell surface antigen; OX40 homologue; CD134 antigen; ATC35 antigen; OX40 antigen; OTTHUMP00000001522; ACT35 antigen; OX40L receptor; TAX transcriptionally-activated glycoprotein 1 receptor
• Gene ID: 7293
• mRNA Refseq: NM_003327
• Protein Refseq: NP_003318
• MIM: 600315
• UniProt ID: P43489
• Chromosome Location: 1p36
• Pathway: Cytokine-cytokine receptor interaction
• Function: protein binding; receptor activity; tumor necrosis factor receptor activity