Recombinant Human TJP3 protein, His & T7-tagged

• Cat.No.: TJP3-1724H
• Product Overview: Recombinant Human TJP3 aa. (Met1~Leu210 (Accession # O95049)) fused with N-terminal His & T7 tag was produced in E. coli cells.

Specification

• Description: The protein encoded by this gene is a member of the membrane-associated guanylate kinase-like (MAGUK) protein family which is characterized by members having multiple PDZ domains, a single SH3 domain, and a single guanylate kinase-like (GUK)-domain. In addition, members of the zonula occludens protein subfamily have an acidic domain, a basic arginine-rich region, and a proline-rich domain. The protein encoded by this gene plays a role in the linkage between the actin cytoskeleton and tight-junctions and also sequesters cyclin D1 at tight junctions during mitosis. Alternative splicing results in multiple transcript variants encoding distinct isoforms. This gene has a partial pseudogene on chromosome 1.
• Source: E. coli
• Species: Human
• Tag: His&T7
• Form: Freeze-dried powder
• Molecular Mass: Predicted Molecular Mass: 26.4kDa
• Protein length: Met1~Leu210
• Endotoxin: <1.0EU per 1ug (determined by the LAL method)
• Purity: >90%
• Applications: SDS-PAGE; WB; ELISA; IP.
• Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
• Storage: Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
• Storage buffer: Supplied as lyophilized form in 20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% sarcosyl, 5% trehalose, and preservative.
• Reconstitution: Reconstitute in sterile ddH2O.
• Isoelectric Point: 10.2

Gene Information

• Gene Name: TJP3 tight junction protein 3 [ Homo sapiens (human) ]
• Official Symbol: TJP3
• Synonyms: TJP3; tight junction protein 3; ZO3; ZO-3; tight junction protein ZO-3; tight junction protein 3 (zona occludens 3); zona occludens protein 3; zonula occludens protein 3
• Gene ID: 27134
• mRNA Refseq: NM_001267560.1
• Protein Refseq: NP_001254489.1
• UniProt ID: O95049