Recombinant Human tight junction protein 1 Protein, His&Flag&StrepII tagged

• Cat.No.: TJP1-015H
• Product Overview: Recombinant human ZO1 protein (Q07157) (Thr 1631-Phe 1748) with a Twin strep tag at the N-terminus and both His, Flag tag at the C-terminus was expressed in E. coli.

Specification

• Description: TJP1, TJP2, and TJP3 are closely related scaffolding proteins that link tight junction (TJ) transmembrane proteins such as claudins, junctional adhesion molecules, and occludin to the actin cytoskeleton. The tight junction acts to limit movement of substances through the paracellular space and as a boundary between the compositionally distinct apical and basolateral plasma membrane domains of epithelial and endothelial cells. Necessary for lumenogenesis, and particularly efficient epithelial polarization and barrier formation. Plays a role in the regulation of cell migration by targeting CDC42BPB to the leading edge of migrating cells. Plays an important role in podosome formation and associated function, thus regulating cell adhesion and matrix remodeling. With TJP2 and TJP3, participates in the junctional retention and stability of the transcription factor DBPA, but is not involved in its shuttling to the nucleus.
• Source: E. coli
• Species: Human
• Tag: N-Twin strep and C-His&Flag
• Protein length: 1631-1748aa
• Molecular Mass: 19.4 kDa
• Endotoxin: Less than 1.0 EU/μg by the LAL method.
• Purity: >95% as determined by SDS-PAGE.
• Storage: Please avoid repeated freeze-thaw cycles. Samples are stable for up to twelve months from date of receipt at -20 to -80 centigrade. It is recommended that aliquot the reconstituted solution to minimize freeze-thaw cycles.
• Storage Buffer: Lyophilized from a 0.2 μm filtered solution of PBS, pH7.4, 5% Trehalose, 5% mannitol.
• Reconstitution: Reconstitute at 250 μg/mL in sterile water.

Gene Information

• Gene Name: TJP1 tight junction protein 1 [ Homo sapiens (human) ]
• Official Symbol: TJP1
• Synonyms: TJP1; tight junction protein 1 (zona occludens 1); tight junction protein ZO-1; DKFZp686M05161; MGC133289; tight junction protein ZO 1; ZO 1; zona occludens 1; zona occludens protein 1; zonula occludens 1 protein; zonula occludens protein 1; ZO-1
• Gene ID: 7082
• mRNA Refseq: NM_003257
• Protein Refseq: NP_003248
• MIM: 601009
• UniProt ID: Q07157