Recombinant Human Thymidine Kinase 1, Soluble, His-tagged

• Cat.No.: TK1-1342H
• Product Overview: Recombinant human TK1 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography.

Specification

• Species: Human
• Source: E.coli
• Tag: His
• Protein Length: 1-234aa
• Description: TK1 is an enzyme, a phosphotransferase (a kinase): 2"-deoxythymidine kinase, ATP-thymidine 5"-phosphotransferase. It is present in two forms in mammalian cells, TK1 and TK2. Thymidine kinases have a key function in the synthesis of DNA and thereby in cell division, as they are part of the unique reaction chain to introduce deoxythymidine into the DNA.
• Form: Liquid. 20mM Tris-HCl buffer (pH8.0) containing 20% glycerol, 0.1M NaCl, 1mM DTT.
• Molecular Mass: 28.0 kDa (258aa) confirmed by MALDI-TOF
• AA Sequence: MGSSHHHHHH SSGLVPRGSH MGSHMSCINL PTVLPGSPSK TRGQIQVILG PMFSGKSTEL MRRVRRFQIA QYKCLVIKYA KDTRYSSSFC THDRNTMEAL PACLLRDVAQ EALGVAVIGI DEGQFFPDIV EFCEAMANAG KTVIVAALDG TFQRKPFGAI LNLVPLAESV VKLTAVCMEC FREAAYTKRL GTEKEVEVIG GADKYHSVCR LCYFKKASGQ PAGPDNKENC PVPGKPGEAV AARKLFAPQQ ILQCSPAN
• Purity: >90% by SDS - PAGE
• Applications: SDS-PAGE
• Storage: Can be stored at 4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -72°C. Avoid repeated freezing and thawing cycles.
• Concentration: 1 mg/ml (determined by Bradford assay)

Gene Information

• Gene Name: TK1 thymidine kinase 1, soluble [ Homo sapiens ]
• Official Symbol: TK1
• Synonyms: TK1; thymidine kinase 1, soluble; thymidine kinase, cytosolic; thymidine kinase-1; thymidine kinase 1 soluble isoform; TK2
• Gene ID: 7083
• mRNA Refseq: NM_003258
• Protein Refseq: NP_003249
• MIM: 188300
• UniProt ID: P04183
• Chromosome Location: 17q23.2-q25.3
• Pathway: Drug metabolism - other enzymes, organism-specific biosystem; Drug metabolism - other enzymes, conserved biosystem; E2F transcription factor network, organism-specific biosystem; Fluoropyrimidine Activity, organism-specific biosystem; Metabolic pathways, organism-specific biosystem; Metabolism, organism-specific biosystem; Metabolism of nucleotides, organism-specific biosystem
• Function: ATP binding; kinase activity; metal ion binding; nucleoside kinase activity; nucleotide binding; thymidine kinase activity; transferase activity; zinc ion binding