Recombinant Human TGFA protein

Cat.No. : TGFA-386H
Product Overview : Recombinant Human TGFA protein(P01135-1) (Val40-Ala89) was expressed and purified in E. coli.
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Source : E. coli
Species : Human
Tag : Non
Protein length : 40-89 a.a.
Form : Lyophilized from sterile 50 mM Tris, 100 mM NaCl, pH 8.0.
Bio-activity : 1.Measured by its binding ability in a functional ELISA. Immobilized Human TGF-alpha at 2 μg/ml (100 μl/well) can bind Human EGFR hFc, the EC50 of Human EGFR hFc is 80-500 ng/mL. 2. Measured in a cell proliferation assay using Balb/c 3T3 mouse embryonic fibroblast cells. The ED50 for this effect is 0.5-2.5 ng/mL.
Molecular Mass : The recombinant human TGFa consists of 50 amino acids and has a predicted molecular mass of 5.6 kDa. The apparent molecular mass of it is approximately 13 kDa in SDS-PAGE under reducing conditions.
Purity : > 90 % as determined by SDS-PAGE
Storage : Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution : It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.
Gene Name TGFA transforming growth factor, alpha [ Homo sapiens ]
Official Symbol TGFA
Synonyms TGFA; transforming growth factor, alpha; protransforming growth factor alpha; TGF-alpha; TFGA;
Gene ID 7039
mRNA Refseq NM_001099691
Protein Refseq NP_001093161
MIM 190170
UniProt ID P01135

Not For Human Consumption!

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