Recombinant Human TGFA protein
Cat.No. : | TGFA-386H |
Product Overview : | Recombinant Human TGFA protein(P01135-1) (Val40-Ala89) was expressed and purified in E. coli. |
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Source : | E. coli |
Species : | Human |
Tag : | Non |
Protein length : | 40-89 a.a. |
Form : | Lyophilized from sterile 50 mM Tris, 100 mM NaCl, pH 8.0. |
Bio-activity : | 1.Measured by its binding ability in a functional ELISA. Immobilized Human TGF-alpha at 2 μg/ml (100 μl/well) can bind Human EGFR hFc, the EC50 of Human EGFR hFc is 80-500 ng/mL. 2. Measured in a cell proliferation assay using Balb/c 3T3 mouse embryonic fibroblast cells. The ED50 for this effect is 0.5-2.5 ng/mL. |
Molecular Mass : | The recombinant human TGFa consists of 50 amino acids and has a predicted molecular mass of 5.6 kDa. The apparent molecular mass of it is approximately 13 kDa in SDS-PAGE under reducing conditions. |
Purity : | > 90 % as determined by SDS-PAGE |
Storage : | Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. |
Reconstitution : | It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents. |
Gene Name | TGFA transforming growth factor, alpha [ Homo sapiens ] |
Official Symbol | TGFA |
Synonyms | TGFA; transforming growth factor, alpha; protransforming growth factor alpha; TGF-alpha; TFGA; |
Gene ID | 7039 |
mRNA Refseq | NM_001099691 |
Protein Refseq | NP_001093161 |
MIM | 190170 |
UniProt ID | P01135 |
Not For Human Consumption!
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