Recombinant Human TDP1, His-tagged

• Cat.No.: TDP1-7250H
• Product Overview: Recombinant human TDP1, fused to His-tag at N-terminus, was expressed in E.coli.

Specification

• Description: Tyrosyl-DNA phosphodiesterase 1, also known as TDP1, is involved in repairing stalled topoisomerase I-DNA complexes by catalyzing the hydrolysis of the phosphodiester bond between the tyrosine residue of topoisomerase I and the 3-prime phosphate of DNA. This protein may also remove glycolate from single-stranded DNA containing 3-prime phosphoglycolate, suggesting a role in repair of free-radical mediated DNA double-strand breaks.
• Source: E. coli
• Species: Human
• Tag: His
• Form: Liquid. In 20mM Tris-HCl buffer (pH 8.0) containing 0.4M urea, 10% glycerol.
• Molecular Mass: 35.8 kDa
• Protein length: 1-298 a.a.
• Purity: > 90% by SDS - PAGE.
• Storage: Can be stored at +4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C. Avoid repeated freezing and thawing cycles.
• Concentration: 1 mg/ml (determined by Bradford assay).

Gene Information

• Gene Name: TDP1 tyrosyl-DNA phosphodiesterase 1 [ Homo sapiens ]
• Official Symbol: TDP1
• Synonyms: TDP1; tyrosyl-DNA phosphodiesterase 1; FLJ11090; SCAN1; tyr-DNA phosphodiesterase 1; MGC104252
• Gene ID: 55775
• mRNA Refseq: NM_001008744
• Protein Refseq: NP_001008744
• MIM: 607198
• UniProt ID: Q9NUW8
• Chromosome Location: 14q32.11
• Pathway: DNA Repair, organism-specific biosystem; Double-Strand Break Repair, organism-specific biosystem; Nonhomologous End-joining (NHEJ), organism-specific biosystem; Processing of DNA ends prior to end rejoining, organism-specific biosystem
• Function: 3-tyrosyl-DNA phosphodiesterase activity; double-stranded DNA binding; exonuclease activity; hydrolase activity; phosphoric diester hydrolase activity; protein binding; single-stranded DNA binding