Recombinant Human TDGF1 protein(Met1-Thr172), hFc-tagged

• Cat.No.: TDGF1-1048H
• Product Overview: Recombinant Human TDGF1 (NP_003203.1) (Met1-Thr172) was expressed in HEK293 with the Fc region of Human IgG1 at the C-terminus.

Specification

• Source: HEK293
• Species: Human
• Tag: Fc
• Protein length: Met1-Thr172
• Form: Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
• Bio-activity: Measured by its binding ability in a functional ELISA. Immobilized ALK4-His at 10 μg/mL (100 μL/well) can bind TDGF1-Fc, the EC50 of human TDGF1-Fc is 6.0-14 ng/mL.
• Molecular Mass: The recombinant human TDGF1 consists of 380 amino acids and predicts a molecular mass of 42.7 kDa.
• Endotoxin: < 1.0 EU per μg protein as determined by the LAL method.
• Purity: > 95 % as determined by SDS-PAGE.
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: TDGF1 teratocarcinoma-derived growth factor 1 [ Homo sapiens ]
• Official Symbol: TDGF1
• Synonyms: TDGF1; teratocarcinoma-derived growth factor 1; CR; CRIPTO; Cripto 1; cripto-1 growth factor; epidermal growth factor-like cripto protein CR1; CRGF
• Gene ID: 6997
• mRNA Refseq: NM_001174136
• Protein Refseq: NP_001167607
• UniProt ID: P13385