Recombinant Human Teratocarcinoma-Derived Growth Factor 1

• Cat.No.: TDGF1-495H
• Product Overview: Recombinant Human teratocarcinoma-derived growth factor 1 encoding the human teratocarcinoma-derived growth factor 1 protein (comprising the signal peptide sequence and the first 139 amino acids of the mature protein, encompassing the EGF-like domain) was expressed in modifiedhuman 293 cells.

Specification

• Species: Human
• Source: HEK293
• Tag: Non
• Description: Teratocarcinoma-derived growth factor 1 is the founding member of the EGF-CFC gene family, which is conserved among vertebrates. Proteins within this family contain a signal sequence, a characteristic EGF-like domain, a cysteine-rich region termed the cryptic (CFC) motif, and a hydrophobic C? terminus. It has been reported that Cripto-1 is O-fucosylated on Thr-88 and that this modification is required for the physical interaction with Nodal as well as for signalling activity. We have confirmed that teratocarcinoma-derived growth factor 1 is O-fucosylated at this site.
• Amino Acid Sequence: LGHQEFARPSRGYLAFRDDSIWPQEEPAIRPRSSQRVPPMGIQHSKELNRTCCLNGGTC MLGSFCACPPSFYGRNCEHDVRKENCGSVPHDTWLPKKCSLCKCWHGQLRCFPQAFL PGCDGLVMDEHLVASRTPELPPS.
• Molecular Mass: Under reducing conditions teratocarcinoma-derived growth factor 1 migrates as a broad band between 20 and 30 kDa on SDS-PAGE due to post-translational modifications, in particular glycosylation. This compares with unmodified Cripto-1 polypeptide that has a predicted monomeric molecular mass of 15.6 kDa.
• pI: Teratocarcinoma-derived growth factor 1 has a predicted pI of 8.04.
• % Carbohydrate: Purified teratocarcinoma-derived growth factor 1 consists of 20-50% carbohydrate by weight.
• Glycosylation: Teratocarcinoma-derived growth factor 1 contains N- and O-linked oligosaccharides with confirmed O-fucosylation at Thr-88.
• Purity: >95%, as determined by SDS-PAGE, visualised by Coomassie Brilliant Blue.
• Formulation: When reconstituted in 0.5 ml sterile phosphate-buffered saline, the solution will contain 1% human serum albumin (HSA) and 10% trehalose.
• Reconstitution: It is recommended that 0.5 ml of sterile phosphate-buffered saline be added to the vial.
• Storage: Lyophilised products should be stored at 2 to 8°C. Following reconstitution short-term storage at 4°C is recommended, with longer-term storage in aliquots at -18 to -20°C. Repeated freeze thawing is not recommended.
• Activity: Teratocarcinoma-derived growth factor 1 has been shown to stimulate MAPK phosphorylation in HUVEC cells. 200ng/ml is sufficient to stimulate phosphorylation.

Gene Information

• Gene Name: TDGF1 teratocarcinoma-derived growth factor 1 [ Homo sapiens ]
• Synonyms: TDGF1; teratocarcinoma-derived growth factor 1; CR; CRGF; CRIPTO; Cripto-1; Epidermal growth factor-like cripto protein CR1; Cripto-1 growth factor
• Gene ID: 6997
• mRNA Refseq: NM_003212
• Protein Refseq: NP_003203
• UniProt ID: P13385
• Chromosome Location: 3p21.31
• MIM: 187395
• Function: growth factor activity