Recombinant Human TBP, His-tagged

• Cat.No.: TBP-30154TH
• Product Overview: Recombinant full length human TATA binding protein TBP with His tag, isolated from an E. coli strain that carries the coding sequence for the protein under the control of T7 promoter.

Specification

• Species: Human
• Source: E.coli
• Tag: His
• Description: Initiation of transcription by RNA polymerase II requires the activities of more than 70 polypeptides. The protein that coordinates these activities is transcription factor IID (TFIID), which binds to the core promoter to position the polymerase properly, serves as the scaffold for assembly of the remainder of the transcription complex, and acts as a channel for regulatory signals. TFIID is composed of the TATA-binding protein (TBP) and a group of evolutionarily conserved proteins known as TBP-associated factors or TAFs. TAFs may participate in basal transcription, serve as coactivators, function in promoter recognition or modify general transcription factors (GTFs) to facilitate complex assembly and transcription initiation. This gene encodes TBP, the TATA-binding protein. A distinctive feature of TBP is a long string of glutamines in the N-terminus. This region of the protein modulates the DNA binding activity of the C terminus, and modulation of DNA binding affects the rate of transcription complex formation and initiation of transcription. The number of CAG repeats encoding the polyglutamine tract is usually 32-39, and expansion of the number of repeats increases the length of the polyglutamine string and is associated with spinocerebellar ataxia 17, a neurodegenerative disorder classified as a polyglutamine disease. Two transcript variants encoding different isoforms have been found for this gene.
• Conjugation: HIS
• Biological activity: 1 unit equals 1 nanogram of purified protein.
• Form: Liquid
• Purity: >95% by SDS-PAGE
• Storage buffer: Preservative: NoneConstituents: 20% Glycerol, 20mM Tris Cl, 100mM Potassium chloride, 1mM DTT, 0.2mM EDTA, pH 8.0
• Storage: Shipped on dry ice. Upon delivery aliquot and store at -80oC. Avoid freeze / thaw cycles.
• Full Length: Full L.

Gene Information

• Gene Name: TBP TATA box binding protein [ Homo sapiens ]
• Official Symbol: TBP
• Synonyms: TBP; TATA box binding protein; GTF2D1, SCA17; TATA-box-binding protein; TFIID
• Gene ID: 6908
• mRNA Refseq: NM_001172085
• Protein Refseq: NP_001165556
• MIM: 600075
• Uniprot ID: P20226
• Chromosome Location: 6q27
• Pathway: Basal transcription factors, organism-specific biosystem; Basal transcription factors, conserved biosystem; Eukaryotic Transcription Initiation, organism-specific biosystem; Formation and Maturation of mRNA Transcript, organism-specific biosystem; Gene Expression, organism-specific biosystem
• Function: DNA binding; binding; protein binding; repressing transcription factor binding; sequence-specific DNA binding transcription factor activity