Active Recombinant Human TATA Box Binding Protein, GST-tagged

• Cat.No.: TBP-1169H

Specification

• Species: Human
• Source: E.coli
• Tag: GST
• Description: The TATA-binding protein (TBP) is believed to function as an essential factor of the general transcription machinery and to be involved in transcription by all three eukaryotic RNA Polymerases (Pol I, II, and III). TBP specifically binds to the TATA element at the promoter region and interacts with numerous transcription factors, including TBP-associated factors (TAFs), activators, and some tumor suppressor proteins. Isolated from anE. colistrain that carries the coding sequence for human TBP under the control of T7 promoter. GST-TBP can be used for protein-protein interaction assay.
• Usage: For in vitro use only.
• Form: Liquid. Supplied in 20 mM Tris-HCl pH 8.0, 100 Mm KCl, 0.2 mM EDTA, 1 mM DTT and 20% glycerol.
• Activity: 100 ng of the protein is sufficient for a protein-protein interaction assay detected by immuno-blot system.
• Purity: > 95% by SDS-PAGE.
• Storage: Quality guaranteed for 12 months, store at -80°C. Avoid freeze / thaw cycles.

Gene Information

• Gene Name: TBP TATA box binding protein [ Homo sapiens ]
• Synonyms: TBP; TATA box binding protein; GTF2D; SCA17; TFIID; GTF2D1; MGC117320; MGC126054; MGC126055; TATA-box binding protein N-terminal domain; TATA-box factor; TATA-binding factor; TATA sequence-binding protein; Transcription initiation factor TFIID TBP subunit
• Gene ID: 6908
• mRNA Refseq: NM_003194
• Protein Refseq: NP_003185
• MIM: 600075
• UniProt ID: P20226V
• Chromosome Location: 6q27
• Pathway: Basal transcription factors; Huntington"s disease; Gene Expression; HIV Infection; Transcription
• Function: general RNA polymerase II transcription factor activity; transcription factor activity