Recombinant Human SUMF2 protein, MYC/DDK-tagged

• Cat.No.: SUMF2-35H
• Product Overview: Recombinant Human SUMF2, transcript variant 2, fused with MYC/DDK tag at C-terminal was expressed in HEK293.

Specification

• Species: Human
• Source: HEK293
• Tag: DDK&Myc
• Description: The catalytic sites of sulfatases are only active if they contain a unique amino acid, C-alpha-formylglycine (FGly). The FGly residue is posttranslationally generated from a cysteine by enzymes with FGly-generating activity. The gene described in this record is a member of the sulfatase-modifying factor family and encodes a protein with a DUF323 domain that localizes to the lumen of the endoplasmic reticulum. This protein has low levels of FGly-generating activity but can heterodimerize with another family member - a protein with high levels of FGly-generating activity. Alternate transcriptional splice variants, encoding different isoforms, have been characterized.
• Form: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol.
• Molecular Mass: 31.2 kDa
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Notes: Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps.
• Concentration: >50 ug/mL as determined by microplate BCA method

Gene Information

• Gene Name: SUMF2 sulfatase modifying factor 2 [ Homo sapiens ]
• Official Symbol: SUMF2
• Synonyms: SUMF2; sulfatase modifying factor 2; sulfatase-modifying factor 2; DKFZp566I1024; C-alpha-formyglycine-generating enzyme 2; C-alpha-formylglycine-generating enzyme 2; paralog of the formylglycine-generating enzyme; pFGE; MGC99485; DKFZp686I1024; DKFZp781L1035; DKFZp686L17160
• Gene ID: 25870
• mRNA Refseq: NM_015411
• Protein Refseq: NP_056226
• MIM: 607940
• UniProt ID: Q8NBJ7
• Chromosome Location: 7q11.1
• Function: binding; metal ion binding