Recombinant Human SNRNP70 Protein, Myc/DDK-tagged, C13 and N15-labeled

• Cat.No.: SNRNP70-1036H
• Product Overview: SNRNP70 MS Standard C13 and N15-labeled recombinant protein (NP_003080) with a C-terminal MYC/DDK tag, was expressed in HEK293 cells.

Specification

• Description: Component of the spliceosomal U1 snRNP, which is essential for recognition of the pre-mRNA 5' splice-site and the subsequent assembly of the spliceosome. SNRNP70 binds to the loop I region of U1-snRNA. The truncated isoforms cannot bind U1-snRNA.
• Source: HEK293
• Species: Human
• Tag: Myc&DDK
• Molecular Mass: 51.6 kDa
• AA Sequence: MTQFLPPNLLALFAPRDPIPYLPPL EKLPHEKHHNQPYCGIAPYIREFED PRDAPPPTRAETREERMERKRREKI ERRQQEVETELKMWDPHNDPNAQGD AFKTLFVARVNYDTTESKLRREFEV YGPIKRIHMVYSKRSGKPRGYAFIE YEHERDMHSAYKHADGKKIDGRRVL VDVERGRTVKGWRPRRLGGGLGGTR RGGADVNIRHSGRDDTSRYDERPGP SPLPHRDRDRDRERERRERSRERDK ERERRRSRSRDRRRRSRSRDKEERR RSRERSKDKDRDRKRRSSRSRERAR RERERKEELRGGGGDMAEPSEAGDA PPDDGPPGELGPDGPDGPEEKGRDR DRERRRSHRSERERRRDRDRDRDRD REHKRGERGSERGRDEARGGGGGQD NGLEGLGNDSRDMYMESEGGDGYLA PENGYLMEAAPETRTRPLEQKLISE EDLAANDILDYKDDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Stability: Stable for 3 months from receipt of products under proper storage and handling conditions.
• Storage: Store at -80 centigrade. Avoid repeated freeze-thaw cycles.
• Concentration: 50 μg/mL as determined by BCA
• Storage Buffer: 100 mM glycine, 25 mM Tris-HCl, pH 7.3.

Gene Information

• Gene Name: SNRNP70 small nuclear ribonucleoprotein U1 subunit 70 [ Homo sapiens (human) ]
• Official Symbol: SNRNP70
• Synonyms: SNRNP70; small nuclear ribonucleoprotein 70kDa (U1); RNPU1Z, RPU1, small nuclear ribonucleoprotein 70kDa (RNP antigen), SNRP70; U1 small nuclear ribonucleoprotein 70 kDa; Snp1; U1 70K; U1 snRNP 70 kDa; RPU1; U1AP; U170K; U1RNP; RNPU1Z; SNRP70; U1-70K
• Gene ID: 6625
• mRNA Refseq: NM_003089
• Protein Refseq: NP_003080
• MIM: 180740
• UniProt ID: P08621

Reviews

02/06/2022

This may include providing detailed protocols, troubleshooting advice, and suggestions for experimental design.

04/27/2019

The manufacturer can provide researchers with high-quality ARMC6 protein, ensuring it is properly purified, characterized, and quality-controlled.

12/17/2018

This collaboration may extend beyond the purchase of the SNRNP70 protein, potentially leading to additional support, joint research projects, or access to expertise in the field.

Q&As

How is SNRNP70 linked to autoimmune diseases?

Autoantibodies against SNRNP70 are frequently found in individuals with autoimmune diseases, serving as a diagnostic marker for conditions like SLE.

Are there specific techniques used to detect anti-SNRNP70 antibodies?

Common methods include immunoprecipitation, enzyme-linked immunosorbent assay (ELISA), and Western blotting.

Can changes in SNRNP70 levels be used to monitor treatment response in autoimmune diseases?

Monitoring anti-SNRNP70 antibody levels during treatment may offer insights into the effectiveness of therapeutic interventions in autoimmune diseases.

How might SNRNP70 be a potential therapeutic target?

Understanding the role of SNRNP70 in autoimmune diseases could lead to the development of targeted therapies aimed at modulating its activity.

Can anti-SNRNP70 antibodies be found in diseases other than SLE?

Yes, anti-SNRNP70 antibodies are also associated with other autoimmune diseases such as mixed connective tissue disease (MCTD) and rheumatoid arthritis.