Recombinant Human SNRNP70 293 Cell Lysate

• Cat.No.: SNRNP70-1620HCL

Specification

• Description: Antigen standard for small nuclear ribonucleoprotein 70kDa (U1) (SNRNP70) is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
• Source: HEK 293 cells
• Species: Human
• Components: This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
• Size: 0.1 mg
• Storage Instruction: Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
• Applications: ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.
• Tag: Non

Gene Information

• Gene Name: SNRNP70 small nuclear ribonucleoprotein 70kDa (U1) [ Homo sapiens ]
• Official Symbol: SNRNP70
• Synonyms: SNRNP70; small nuclear ribonucleoprotein 70kDa (U1); RNPU1Z, RPU1, small nuclear ribonucleoprotein 70kDa (RNP antigen) , SNRP70; U1 small nuclear ribonucleoprotein 70 kDa; Snp1; U1 70K; U1 snRNP 70 kDa; RPU1; U1AP; U170K; U1RNP; RNPU1Z; SNRP70; U1-70K
• Gene ID: 6625
• mRNA Refseq: NM_003089
• Protein Refseq: NP_003080
• MIM: 180740
• UniProt ID: P08621
• Chromosome Location: 19q13.3
• Pathway: Gene Expression, organism-specific biosystem; Processing of Capped Intron-Containing Pre-mRNA, organism-specific biosystem; Spliceosome, organism-specific biosystem; Spliceosome, conserved biosystem; Spliceosome, U1-snRNP, organism-specific biosystem; mRNA Splicing, organism-specific biosystem; mRNA Splicing - Major Pathway, organism-specific biosystem
• Function: RNA binding; nucleotide binding; protein binding

Reviews

02/06/2022

This may include providing detailed protocols, troubleshooting advice, and suggestions for experimental design.

04/27/2019

The manufacturer can provide researchers with high-quality ARMC6 protein, ensuring it is properly purified, characterized, and quality-controlled.

12/17/2018

This collaboration may extend beyond the purchase of the SNRNP70 protein, potentially leading to additional support, joint research projects, or access to expertise in the field.

Q&As

How is SNRNP70 linked to autoimmune diseases?

Autoantibodies against SNRNP70 are frequently found in individuals with autoimmune diseases, serving as a diagnostic marker for conditions like SLE.

Are there specific techniques used to detect anti-SNRNP70 antibodies?

Common methods include immunoprecipitation, enzyme-linked immunosorbent assay (ELISA), and Western blotting.

Can changes in SNRNP70 levels be used to monitor treatment response in autoimmune diseases?

Monitoring anti-SNRNP70 antibody levels during treatment may offer insights into the effectiveness of therapeutic interventions in autoimmune diseases.

How might SNRNP70 be a potential therapeutic target?

Understanding the role of SNRNP70 in autoimmune diseases could lead to the development of targeted therapies aimed at modulating its activity.

Can anti-SNRNP70 antibodies be found in diseases other than SLE?

Yes, anti-SNRNP70 antibodies are also associated with other autoimmune diseases such as mixed connective tissue disease (MCTD) and rheumatoid arthritis.