Recombinant Human SNRNP70 Protein, His-tagged

• Cat.No.: SNRNP70-2711H
• Product Overview: Recombinant Human SNRNP70 Protein (Met1-Glu437) with a N-His tag was expressed in E. coli.

Specification

• Description: Enables U1 snRNA binding activity. Involved in mRNA splicing, via spliceosome and regulation of RNA splicing. Located in nucleoplasm. Part of U1 snRNP and spliceosomal complex. Implicated in disease of mental health and systemic lupus erythematosus. Biomarker of Alzheimer's disease.
• Source: E. coli
• Species: Human
• Tag: His
• Form: Freeze-dried powder
• Molecular Mass: Predicted Molecular Mass: 55.3 kDa
  Accurate Molecular Mass: 44 kDa
• Protein length: Met1-Glu437
• Endotoxin: <1.0 EU per 1µg (determined by the LAL method).
• Purity: > 90%
• Applications: Positive Control; Immunogen; SDS-PAGE; WB.
• Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 centigrade for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
• Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 centigrade for one month. Aliquot and store at -80 centigrade for 12 months.
• Storage Buffer: 20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose and Proclin300.
• Reconstitution: Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

Gene Information

• Gene Name: SNRNP70 small nuclear ribonucleoprotein 70kDa (U1) [ Homo sapiens (human) ]
• Official Symbol: SNRNP70
• Synonyms: SNRNP70; small nuclear ribonucleoprotein 70kDa (U1); RNPU1Z, RPU1, small nuclear ribonucleoprotein 70kDa (RNP antigen), SNRP70; U1 small nuclear ribonucleoprotein 70 kDa; Snp1; U1 70K; U1 snRNP 70 kDa; RPU1; U1AP; U170K; U1RNP; RNPU1Z; SNRP70; U1-70K
• Gene ID: 6625
• mRNA Refseq: NM_003089
• Protein Refseq: NP_003080
• MIM: 180740
• UniProt ID: P08621

Reviews

02/06/2022

This may include providing detailed protocols, troubleshooting advice, and suggestions for experimental design.

04/27/2019

The manufacturer can provide researchers with high-quality ARMC6 protein, ensuring it is properly purified, characterized, and quality-controlled.

12/17/2018

This collaboration may extend beyond the purchase of the SNRNP70 protein, potentially leading to additional support, joint research projects, or access to expertise in the field.

Q&As

How is SNRNP70 linked to autoimmune diseases?

Autoantibodies against SNRNP70 are frequently found in individuals with autoimmune diseases, serving as a diagnostic marker for conditions like SLE.

Are there specific techniques used to detect anti-SNRNP70 antibodies?

Common methods include immunoprecipitation, enzyme-linked immunosorbent assay (ELISA), and Western blotting.

Can changes in SNRNP70 levels be used to monitor treatment response in autoimmune diseases?

Monitoring anti-SNRNP70 antibody levels during treatment may offer insights into the effectiveness of therapeutic interventions in autoimmune diseases.

How might SNRNP70 be a potential therapeutic target?

Understanding the role of SNRNP70 in autoimmune diseases could lead to the development of targeted therapies aimed at modulating its activity.

Can anti-SNRNP70 antibodies be found in diseases other than SLE?

Yes, anti-SNRNP70 antibodies are also associated with other autoimmune diseases such as mixed connective tissue disease (MCTD) and rheumatoid arthritis.