Recombinant Human SNAP25 Protein, MYC/DDK-tagged, C13 and N15-labeled

• Cat.No.: SNAP25-181H
• Product Overview: SNAP25 MS Standard C13 and N15-labeled recombinant protein (NP_570824) with a C-terminal MYC/DDK tag, was expressed in HEK293 cells.

Specification

• Description: Synaptic vesicle membrane docking and fusion is mediated by SNAREs (soluble N-ethylmaleimide-sensitive factor attachment protein receptors) located on the vesicle membrane (v-SNAREs) and the target membrane (t-SNAREs). The assembled v-SNARE/t-SNARE complex consists of a bundle of four helices, one of which is supplied by v-SNARE and the other three by t-SNARE. For t-SNAREs on the plasma membrane, the protein syntaxin supplies one helix and the protein encoded by this gene contributes the other two. Therefore, this gene product is a presynaptic plasma membrane protein involved in the regulation of neurotransmitter release. Two alternative transcript variants encoding different protein isoforms have been described for this gene. [provided by RefSeq, Jul 2008]
• Source: HEK293
• Species: Human
• Tag: Myc&DDK
• Molecular Mass: 23.1 kDa
• AA Sequence: MAEDADMRNELEEMQRRADQLADESLESTRRMLQLVEESKDAGIRTLVMLDEQGEQLERIEEGMDQINKDMKEAEKNLTDLGKFCGLCVCPCNKLKSSDAYKKAWGNNQDGVVASQPARVVDEREQMAISGGFIRRVTNDARENEMDENLEQVSGIIGNLRHMALDMGNEIDTQNRQIDRIMEKADSNKTRIDEANQRATKMLGSGTRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Stability: Stable for 3 months from receipt of products under proper storage and handling conditions.
• Storage: Store at -80 centigrade. Avoid repeated freeze-thaw cycles.
• Concentration: 50 μg/mL as determined by BCA
• Storage Buffer: 100 mM glycine, 25 mM Tris-HCl, pH 7.3.

Gene Information

• Gene Name: SNAP25 synaptosomal-associated protein, 25kDa [ Homo sapiens (human) ]
• Official Symbol: SNAP25
• Synonyms: SNAP25; synaptosomal-associated protein, 25kDa; SNAP, synaptosomal associated protein, 25kD; synaptosomal-associated protein 25; bA416N4.2; dJ1068F16.2; resistance to inhibitors of cholinesterase 4 homolog; RIC 4; RIC4; SEC9; SNAP 25; SUP; super protein; SNAP; RIC-4; SNAP-25; FLJ23079
• Gene ID: 6616
• mRNA Refseq: NM_130811
• Protein Refseq: NP_570824
• MIM: 600322
• UniProt ID: P60880