Recombinant Human SMURF1 Protein, Myc/DDK-tagged, C13 and N15-labeled

• Cat.No.: SMURF1-1344H
• Product Overview: SMURF1 MS Standard C13 and N15-labeled recombinant protein (NP_851994) with a C-terminal MYC/DDK tag, was expressed in HEK293 cells.

Specification

• Description: This gene encodes a ubiquitin ligase that is specific for receptor-regulated SMAD proteins in the bone morphogenetic protein (BMP) pathway. This protein plays a key roll in the regulation of cell motility, cell signalling, and cell polarity. Alternative splicing results in multiple transcript variants encoding different isoforms.
• Source: HEK293
• Species: Human
• Tag: Myc&DDK
• Molecular Mass: 83.3 kDa
• AA Sequence: MSNPGTRRNGSSIKIRLTVLCAKNL AKKDFFRLPDPFAKIVVDGSGQCHS TDTVKNTLDPKWNQHYDLYVGKTDS ITISVWNHKKIHKKQGAGFLGCVRL LSNAISRLKDTGYQRLDLCKLNPSD TDAVRGQIVVSLQTRDRIGTGGSVV DCRGLLENEGTVYEDSGPGRPLSCF MEEPAPYTDSTGAAAGGGNCRFVES PSQDQRLQAQRLRNPDVRGSLQTPQ NRPHGHQSPELPEGYEQRTTVQGQV YFLHTQTGVSTWHDPRIPRDLNSVN CDELGPLPPGWEVRSTVSGRIYFVD HNNRTTQFTDPRLHHIMNHQCQLKE PSQPLPLPSEGSLEDEELPAQRYER DLVQKLKVLRHELSLQQPQAGHCRI EVSREEIFEESYRQIMKMRPKDLKK RLMVKFRGEEGLDYGGVAREWLYLL CHEMLNPYYGLFQYSTDNIYMLQIN PDSSINPDHLSYFHFVGRIMGLAVF HGHYINGGFTVPFYKQLLGKPIQLS DLESVDPELHKSLVWILENDITPVL DHTFCVEHNAFGRILQHELKPNGRN VPVTEENKKEYVRLYVNWRFMRGIE AQFLALQKGFNELIPQHLLKPFDQK ELELIIGGLDKIDLNDWKSNTRLKH CVADSNIVRWFWQAVETFDEERRAR LLQFVTGSTRVPLQGFKALQGSTGA AGPRLFTIHLIDANTDNLPKAHTCF NRIDIPPYESYEKLYEKLLTAVEET CGFAVETRTRPLEQKLISEEDLAAN DILDYKDDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Stability: Stable for 3 months from receipt of products under proper storage and handling conditions.
• Storage: Store at -80 centigrade. Avoid repeated freeze-thaw cycles.
• Concentration: 50 μg/mL as determined by BCA
• Storage Buffer: 100 mM glycine, 25 mM Tris-HCl, pH 7.3.

Gene Information

• Gene Name: SMURF1 SMAD specific E3 ubiquitin protein ligase 1 [ Homo sapiens (human) ]
• Official Symbol: SMURF1
• Synonyms: SMURF1; SMAD specific E3 ubiquitin protein ligase 1; E3 ubiquitin-protein ligase SMURF1; KIAA1625; hSMURF1; E3 ubiquitin ligase SMURF1; Smad-specific E3 ubiquitin ligase 1; Smad ubiquitination regulatory factor 1; SMAD-specific E3 ubiquitin-protein ligase 1
• Gene ID: 57154
• mRNA Refseq: NM_181349
• Protein Refseq: NP_851994
• MIM: 605568
• UniProt ID: Q9HCE7