Recombinant Human SMARCB1 Protein (2-376 aa), His-tagged

• Cat.No.: SMARCB1-2740H
• Product Overview: Recombinant Human SMARCB1 Protein (2-376 aa) is produced by Baculovirus expression system. This protein is fused with a 6xHis tag at the N-terminal. Research Area: Cell Cycle. Protein Description: Partial.

Specification

• Description: Core component of the BAF (hSWI/SNF) complex. This ATP-dependent chromatin-rodeling complex plays important roles in cell proliferation and differentiation, in cellular antiviral activities and inhibition of tumor formation. The BAF complex is able to create a stable, altered form of chromatin that constrains fewer negative supercoils than normal. This change in supercoiling would be due to the conversion of up to one-half of the nucleosomes on polynucleosomal arrays into asymmetric structures, termed altosomes, each composed of 2 histones octamers. Stimulates in vitro the rodeling activity of SMARCA4/BRG1/BAF190A. Involved in activation of CSF1 promoter. Belongs to the neural progenitors-specific chromatin rodeling complex (npBAF complex) and the neuron-specific chromatin rodeling complex (nBAF complex). During neural development a switch from a st/progenitor to a post-mitotic chromatin rodeling mechanism occurs as neurons exit the cell cycle and become committed to their adult state. The transition from proliferating neural st/progenitor cells to post-mitotic neurons requires a switch in subunit composition of the npBAF and nBAF complexes. As neural progenitors exit mitosis and differentiate into neurons, npBAF complexes which contain ACTL6A/BAF53A and PHF10/BAF45A, are exchanged for homologous alternative ACTL6B/BAF53B and DPF1/BAF45B or DPF3/BAF45C subunits in neuron-specific complexes (nBAF). The npBAF complex is essential for the self-renewal/proliferative capacity of the multipotent neural st cells. The nBAF complex along with CREST plays a role regulating the activity of genes essential for dendrite growth . Plays a key role in cell-cycle control and causes cell cycle arrest in G0/G1.
• Source: Baculovirus
• Species: Human
• Tag: His
• Form: Tris-based buffer,50% glycerol
• Molecular Mass: 45.0 kDa
• Protein length: 2-376 aa
• AA Sequence: MMMALSKTFGQKPVKFQLEDDGEFY MIGSEVGNYLRMFRGSLYKRYPSLW RRLATVEERKKIVASSHGKKTKPNT KDHGYTTLATSVTLLKASEVEEILD GNDEKYKAVSISTEPPTYLREQKAK RNSQWVPTLPNSSHHLDAVPCSTTI NRNRMGRDKKRTFPLCFDDHDPAVI HENASQPEVLVPIRLDMEIDGQKLR DAFTWNMNEKLMTPEMFSEILCDDL DLNPLTFVPAIASAIRQQIESYPTD SILEDQSDQRVIIKLNIHVGNISLV DQFEWDMSEKENSPEKFALKLCSEL GLGGEFVTTIAYSIRGQLSWHQKTY AFSENPLPTVEIAIRNTGDADQWCP LLETLTDAEMEKKIRDQDRNTRRMR
• Purity: > 90% as determined by SDS-PAGE.
• Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4 centigrade for up to one week.
• Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20 centigrade/-80 centigrade. The shelf life of lyophilized form is 12 months at -20 centigrade/-80 centigrade.
• Concentration: A hardcopy of COA will be sent along with the products. Please refer to it for detailed information.

Gene Information

• Gene Name: SMARCB1 SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily b, member 1 [ Homo sapiens ]
• Official Symbol: SMARCB1
• Synonyms: SMARCB1; SNF5L1; BAF47; hSNFS; Ini1; integrase interactor 1; RDT; Sfh1p; Snr1; homolog like 1; hSNF5; SNF5 homolog; INI1; SNF5; RTPS1
• Gene ID: 6598
• mRNA Refseq: NM_001007468
• Protein Refseq: NP_001007469
• MIM: 601607
• UniProt ID: Q12824