Recombinant Human SMARCA2 Protein (1367-1511), N-GST tagged

• Cat.No.: SMARCA2-13H
• Product Overview: The peptide corresponding to amino acids 1367-1511 that contains the bromodomain sequences of SMARCA2/BRM (accession number NP_003061.3) was expressed in E. coli and contains an N-terminal GST-Tag with an observed molecular weight of 44.2 kDa. It shows binding specificity for acetylated H3K9, H3K14, H3K9/14, H4K8, H4K12, H4K16 and H4K5/8/12/16.

Specification

• Description: The protein encoded by this gene is a member of the SWI/SNF family of proteins and is highly similar to the brahma protein of Drosophila. Members of this family have helicase and ATPase activities and are thought to regulate transcription of certain genes by altering the chromatin structure around those genes. The encoded protein is part of the large ATP-dependent chromatin remodeling complex SNF/SWI, which is required for transcriptional activation of genes normally repressed by chromatin. Alternatively spliced transcript variants encoding different isoforms have been found for this gene, which contains a trinucleotide repeat (CAG) length polymorphism.
• Source: E. coli
• Species: Human
• Tag: GST
• Molecular Mass: 44.2 kDa
• Protein length: 1367-1511
• Purity: The recombinant protein is >95% pure by SDS-PAGE.
• Applications: Recombinant SMARCA2/BRM (1367-1511), GST-Tag is suitable for use in binding assays, inhibitor screening, and selectivity profiling.
• Storage: Recombinant proteins in solution are temperature sensitive and must be stored at -80 centigrade to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.
• Concentration: 3.5 mg/mL
• Storage Buffer: 25 mM Tris pH 7.4, 150 mM NaCI and 5% glycerol

Gene Information

• Gene Name: SMARCA2 SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily a, member 2 [ Homo sapiens (human) ]
• Official Symbol: SMARCA2
• Synonyms: SMARCA2; SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily a, member 2; BIS; BRM; SNF2; SWI2; hBRM; NCBRS; Sth1p; BAF190; SNF2L2; SNF2LA; hSNF2a; probable global transcription activator SNF2L2; ATP-dependent helicase SMARCA2; BAF190B; BRG1-associated factor 190B; SNF2-alpha; SNF2/SWI2-like protein 2; SWI/SNF-related matrix-associated actin-dependent regulator of chromatin a2; brahma homolog; global transcription activator homologous sequence; protein brahma homolog; putative global transcription activator SNF2L2; sucrose nonfermenting 2-like protein 2
• Gene ID: 6595
• mRNA Refseq: NM_003070
• Protein Refseq: NP_003061
• MIM: 600014
• UniProt ID: P51531