Recombinant Human SMARCA2 Protein, GST-tagged

• Cat.No.: SMARCA2-195H
• Product Overview: Human recombinant SMARCA2a bromodomain (residues 1367-1511) expressed as an N-terminal GST-fusion protein in E. coli.

Specification

• Description: The protein encoded by this gene is a member of the SWI/SNF family of proteins and is highly similar to the brahma protein of Drosophila. Members of this family have helicase and ATPase activities and are thought to regulate transcription of certain genes by altering the chromatin structure around those genes. The encoded protein is part of the large ATP-dependent chromatin remodeling complex SNF/SWI, which is required for transcriptional activation of genes normally repressed by chromatin. Alternatively spliced transcript variants encoding different isoforms have been found for this gene, which contains a trinucleotide repeat (CAG) length polymorphism.
• Source: E. coli
• Species: Human
• Tag: GST
• Molecular Mass: 43.9 kDa
• Protein length: 1367-1511
• Purity: >95% by SDS-PAGE
• Storage: -70 centigrade. Thaw quickly and store on ice before use. The remaining, unused, undiluted protein should be snap frozen, for example in a dry/ice ethanol bath or liquid nitrogen. Minimize freeze/thaws if possible, but very low volume aliquots (<5 μL) or storage of diluted protein is not recommended.
• Storage Buffer: Solution of purified recombinant protein in 50 mM Tris/HCl, pH 7.5, 500 mM NaCl, 1 mM TCEP, 10% glycerol (v/v)

Gene Information

• Gene Name: SMARCA2 SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily a, member 2 [ Homo sapiens (human) ]
• Official Symbol: SMARCA2
• Synonyms: SMARCA2; SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily a, member 2; BRM; SNF2; SWI2; hBRM; NCBRS; Sth1p; BAF190; SNF2L2; SNF2LA; hSNF2a; probable global transcription activator SNF2L2; ATP-dependent helicase SMARCA2; BAF190B; BRG1-associated factor 190B; SNF2-alpha; SNF2/SWI2-like protein 2; SWI/SNF-related matrix-associated actin-dependent regulator of chromatin a2; brahma homolog; global transcription activator homologous sequence; protein brahma homolog; sucrose nonfermenting 2-like protein 2
• Gene ID: 6595
• mRNA Refseq: NM_003070
• Protein Refseq: NP_003061
• MIM: 600014
• UniProt ID: P51531