Recombinant Human SIRT2 Protein, His-tagged

• Cat.No.: SIRT2-23H
• Product Overview: Recombinant human SIRT2(2-389) protein with a N-terminal His-tag was expressed in E. coli.

Specification

• Description: This gene encodes a member of the sirtuin family of proteins, homologs to the yeast Sir2 protein. Members of the sirtuin family are characterized by a sirtuin core domain and grouped into four classes. The functions of human sirtuins have not yet been determined; however, yeast sirtuin proteins are known to regulate epigenetic gene silencing and suppress recombination of rDNA. Studies suggest that the human sirtuins may function as intracellular regulatory proteins with mono-ADP-ribosyltransferase activity. The protein encoded by this gene is included in class I of the sirtuin family. Several transcript variants are resulted from alternative splicing of this gene.
• Source: E. coli
• Species: Human
• Tag: His
• Molecular Mass: 44.2 kDa
• Protein length: 2-389
• Purity: ≥90% estimated by SDS-PAGE
• Unit Definition: One unit is defined as the amount of enzyme required to produce 1 nmol of 7-amino-4-methylcouarin per minute at 25 centigrade in 50 mM Tris-HCl, pH 8.0, 137 mM NaCl, 2.7 mM KCl, 1 mM MgCl2, containing 125 μM p53 amino acids 317-320 (Gln-Pro-Lys-Lys(e-acetyl)-AMC), and 6 mM NAD+.
• Stability: ≥ 1 year
• Storage: Store it at -80 centigrade
• Storage Buffer: 50 mM sodium phosphate, pH 7.2, containing 100 mM sodium chloride and 20% glycerol

Gene Information

• Gene Name: SIRT2 sirtuin 2 [ Homo sapiens (human) ]
• Official Symbol: SIRT2
• Synonyms: SIRT2; sirtuin 2; SIR2; SIR2L; SIR2L2; NAD-dependent protein deacetylase sirtuin-2; NAD-dependent deacetylase sirtuin-2; SIR2-like protein 2; regulatory protein SIR2 homolog 2; silent information regulator 2; sir2-related protein type 2; sirtuin type 2
• Gene ID: 22933
• mRNA Refseq: NM_001193286
• Protein Refseq: NP_001180215
• MIM: 604480
• UniProt ID: Q8IXJ6

Reviews

04/15/2021

Efficient and knowledgeable customer service—made our purchase straightforward.

11/02/2020

This recombined protein is our go-to for dependable and accurate results.

08/30/2018

Punctual delivery is crucial, and this product arrived right on time.

Q&As

How does SIRT2 participate in the regulation of cellular stress responses?

SIRT2 is involved in stress response pathways, deacetylating and modulating the activity of stress-responsive proteins, contributing to cellular adaptation to various stress conditions.

How does SIRT2 contribute to the regulation of cellular metabolism?

SIRT2 influences cellular metabolism by deacetylating enzymes involved in glycolysis, fatty acid metabolism, and mitochondrial function, impacting overall energy homeostasis.

Explain the molecular mechanisms underlying SIRT2-mediated deacetylation of target proteins.

SIRT2 uses NAD+ as a cofactor to remove acetyl groups from lysine residues, a process that links cellular metabolic state to protein acetylation and deacetylation.

Discuss the role of SIRT2 in the regulation of cell cycle progression.

SIRT2 influences cell cycle progression by deacetylating key regulators, such as cyclin-dependent kinases (CDKs) and checkpoint proteins, impacting cell cycle checkpoints and progression.

Discuss the role of SIRT2 in neurodegenerative diseases and its potential as a therapeutic target.

SIRT2 has been implicated in neurodegenerative diseases, and modulating its activity is explored as a potential therapeutic strategy for conditions such as Parkinson's disease.