Recombinant Human SIRPB1 protein, His-tagged

• Cat.No.: SIRPB1-5658H
• Product Overview: Recombinant Human SIRPB1 aa. (Ser127~Gly356) fused with N-terminal His tag was produced in E. coli cells.

Specification

• Species: Human
• Source: E.coli
• Tag: His
• Protein Length: Ser127~Gly356
• Description: The protein encoded by this gene is a member of the signal-regulatory-protein (SIRP) family, and also belongs to the immunoglobulin superfamily. SIRP family members are receptor-type transmembrane glycoproteins known to be involved in the negative regulation of receptor tyrosine kinase-coupled signaling processes. This protein was found to interact with TYROBP/DAP12, a protein bearing immunoreceptor tyrosine-based activation motifs. This protein was also reported to participate in the recruitment of tyrosine kinase SYK. Multiple transcript variants encoding different isoforms have been found for this gene.
• Form: Freeze-dried powder
• Molecular Mass: 32kDa as determined by SDS-PAGE reducing conditions.
• Endotoxin: <1.0EU per 1µg (determined by the LAL method)
• Purity: >95%
• Characteristic: The isoelectric point is 6.
• Applications: SDS-PAGE; WB; ELISA; IP; CoIP; Purification; Amine Reactive Labeling.
• Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
• Storage: Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
• Concentration: 200μg/mL
• Storage buffer: 100mM NaHCO3, 500mM NaCl, pH8.3, containing 1mM EDTA, 1mM DTT, 0.01% sarcosyl, 5%Trehalose and Proclin300.
• Reconstitution: Reconstitute in 100mM NaHCO3, 500mM NaCl (pH8.3) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

Gene Information

• Gene Name: SIRPB1 signal regulatory protein beta 1 [ Homo sapiens (human) ]
• Official Symbol: SIRPB1
• Synonyms: SIRPB1; signal regulatory protein beta 1; CD172b; SIRP-BETA-1; signal-regulatory protein beta-1; CD172 antigen-like family member B
• Gene ID: 10326
• mRNA Refseq: NM_001083910.3
• Protein Refseq: NP_001077379.1
• UniProt ID: O00241