Recombinant Human SIRPB1 protein, Fc-tagged

• Cat.No.: SIRPB1-345H
• Product Overview: Recombinant Human SIRPB1 protein (Glu30-Leu371), fused to human IgG1 Fc tag at C-terminus, was expressed in human 293 cells (HEK293).

Specification

• Description: Signal-regulatory protein beta-1 (SIRP beta 1) also known as SIRPβ1, belongs to signal-regulatory-protein (SIRP) family, and immunoglobulin superfamily. SIRPβ1 is a transmembrane protein that has three Ig-like domains in its extracellular region and a short cytoplasmic tail. Human SIRPβ1 is expressed on monocytes and granulocytes but not on lymphocytes. SIRP beta 1 contains short cytoplasmic domains that lack cytoplasmic sequence motifs capable of recruiting SHP-2 and SHP-1. In addition, they contain a single basic lysine residue within the hydrophobic transmembrane domain.
• Source: HEK293
• Species: Human
• Tag: Fc
• Form: Lyophilized from 0.22 um filtered solution in Tris with Glycine, Arginine and NaCl, pH7.5, 10% trehalose.
• Molecular Mass: The protein has a calculated MW of 63.7 kDa. The protein migrates as 70-80 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
• Protein length: 342
• Endotoxin: Less than 1.0 EU per ug by the LAL method.
• Purity: >90% as determined by SDS-PAGE.
• Storage: For long term storage, the product should be stored at lyophilized state at -20 centigrade or lower.
  Please avoid repeated freeze-thaw cycles.
  This product is stable after storage at:
  -20 centigrade to -70 centigrade for 12 months in lyophilized state;
  -70 centigrade for 3 months under sterile conditions after reconstitution.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4℃ before opening to recover the entire contents.

Gene Information

• Gene Name: SIRPB1
• Official Symbol: SIRPB1
• Synonyms: SIRPB1; signal-regulatory protein beta 1; signal-regulatory protein beta-1; CD172b; SIRP BETA 1; CD172 antigen-like family member B; SIRP-BETA-1; FLJ26614; DKFZp686A05192
• Gene ID: 10326
• mRNA Refseq: NM_001083910
• Protein Refseq: NP_001077379
• MIM: 603889
• UniProt ID: O00241