Recombinant Human SET And MYND Domain Containing 2, His-tagged

• Cat.No.: SMYD2-921H
• Product Overview: Recombinant Human SMYD3 protein (Lys 35 - Ser 369), fused with the GST tag at the N-terminus, was expressed inInsect cells.

Specification

• Species: Human
• Source: Insect Cells
• Tag: His
• Protein Length: 35-369 a.a.
• Description: SET and MYND domain-containing protein 3, also known as Zinc finger MYND domain-containing protein 1, SMYD3, and ZMYND, is a member of the histone-lysine methyltransferase family. SMYD3 contains one MYND-type zinc finger and one SET domain. SMYD3 is a histone H3 lysine-4-specific methyltransferase. It is expressed in skeletal muscles and testis. It is overexpressed in a majority of colorectal carcinoma ( CRC ) and hepatocellular carcinoma ( HCC ). SMYD3 plays an important role in transcriptional regulation in human carcinogenesis. It activates the transcription of a set of downstream genes. Of these downstream genes, there are several oncogenes and genes associated with cell adhesion (including those of N-Myc, CrkL, Wnt10b, L-selectin, CD31 and galectin-4), which have been shown to have effects on cell viability, adhesion, migration and metastasis.
• Molecular Mass: The recombinant human SMYD3 / GST chimera consists of 559 amino acids and predicts a molecular mass of 656kDa. It migrates as an approxiamtely 58 KDa band in SDS-PAGE under reducing conditions.
• Predicted N Terminal: Met.
• Purity: > 88 % as determined by SDS-PAGE.
• Formulation: Lyophilized from sterile 20mM Tris,150mM NaCl, 0.5mM DTT, 0.5mM GSH , pH 8.0.
• Endotoxin: < 1.0 EU per 1μg of the protein as determined by the LAL method.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
• Storage And Stability: Samples are stable for up to twelve months from date of receipt at -70⁰C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

Gene Information

• Gene Name: SMYD3 SET and MYND domain containing 3 [ Homo sapiens ]
• Synonyms: SET and MYND domain containing 3; KMT3E; ZMYND1; ZNFN3A1; FLJ21080; MGC104324; bA74P14.1; SMYD3; bA74P14.1 (novel protein); zinc finger, MYND domain containing 1; zinc finger protein, subfamily 3A (MYND domain containing), 1; EC 2.1.1.43; Zinc finger MYND domain-containing protein 1
• Gene ID: 64754
• mRNA Refseq: NM_001167740
• Protein Refseq: NP_001161212
• MIM: 608783
• UniProt ID: Q9H7B4
• Chromosome Location: 1q44
• Function: histone-lysine N-methyltransferase activity; metal ion binding; methyltransferase activity; protein binding; transferase activity; zinc ion binding