Recombinant Mouse Smyd2 Protein, Myc/DDK-tagged

• Cat.No.: Smyd2-5986M
• Product Overview: Purified recombinant protein of mouse full-length SET and MYND domain containing 2 (Smyd2), with C-terminal MYC/DDK tag, expressed in HEK293T cells.

Specification

• Species: Mouse
• Source: HEK293
• Tag: DDK&Myc
• Description: Protein-lysine N-methyltransferase that methylates both histones and non-histone proteins, including p53/TP53 and RB1. Specifically trimethylates histone H3 'Lys-4' (H3K4me3) in vivo. The activity requires interaction with HSP90alpha. Shows even higher methyltransferase activity on p53/TP53. Monomethylates 'Lys-370' of p53/TP53, leading to decreased DNA-binding activity and subsequent transcriptional regulation activity of p53/TP53. Monomethylates RB1 at 'Lys-860'.
• Molecular Mass: 49.6 kDa
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade after receiving vials.
• Concentration: >50 μg/mL as determined by microplate BCA method
• Storage Buffer: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol.

Gene Information

• Gene Name: Smyd2 SET and MYND domain containing 2 [ Mus musculus (house mouse) ]
• Official Symbol: Smyd2
• Synonyms: SMYD2; SET and MYND domain containing 2; N-lysine methyltransferase SMYD2; histone methyltransferase SMYD2; SET and MYND domain-containing protein 2; KMT3C; Zmynd14; 4930402C15; 1110020E07Rik
• Gene ID: 226830
• mRNA Refseq: NM_026796
• Protein Refseq: NP_081072
• UniProt ID: Q8R5A0