Recombinant Human SMYD2 protein, GST-tagged

• Cat.No.: SMYD2-262H
• Product Overview: Recombinant Human SMYD2 fused with GST tag at N-terminal was expressed in E. coli.

Specification

• Species: Human
• Source: E.coli
• Tag: GST
• Description: SMYD2 is a lysine mono-methyltransferase that has been shown to have activity on histone H3, p53 (Lys370), pRB (Lys380) and HSP90 (Lys616). The SMYD2 locus is amplified in lung carcinoma, serous ovarian carcinoma, and pancreatic ductal adenocarcinoma. SMYD2 expression is linked to a variety of cancers. Elevated levels of SMYD2 in esophageal squamous cell carcinoma patients are correlated with lower rates of survival.
• Form: In 100mM Tris pH 8.0, 10 mM glutathione and 10% glycerol.
• Molecular Mass: 68 kDa
• Applications: Recombinant SMYD2, human is useful for histone H3 methylation experiments, enzyme kinetics and inhibitor screening. Use of 1-3 µg SMYD2 per reaction with recombinant histone H3 as a substrate is recommended.
• Storage: Stable for six months at -80 centigrade from date of receipt. For best results, aliquot and avoid multiple freeze/thaws.
• Concentration: 1 µg/µl

Gene Information

• Gene Name: SMYD2 SET and MYND domain containing 2 [ Homo sapiens ]
• Official Symbol: SMYD2
• Synonyms: SMYD2; SET and MYND domain containing 2; N-lysine methyltransferase SMYD2; HSKM B; KMT3C; ZMYND14; lysine N-methyltransferase 3C; histone methyltransferase SMYD2; zinc finger, MYND domain containing 14; SET and MYND domain-containing protein 2; HSKM-B; MGC119305
• Gene ID: 56950
• mRNA Refseq: NM_020197
• Protein Refseq: NP_064582
• MIM: 610663
• UniProt ID: Q9NRG4
• Chromosome Location: 1q32.3
• Pathway: p53 pathway, organism-specific biosystem