Recombinant Human SERPIND1 protein, His-tagged

• Cat.No.: SERPIND1-3157H
• Product Overview: Recombinant Human SERPIND1 (Gly20-Ser499) protein was fused to His-tag at C-terminus and expressed in human 293 cells (HEK293).

Specification

• Species: Human
• Source: HEK293
• Tag: His
• Protein Length: Gly20-Ser499
• Description: Serpin D1 is also known as Heparin cofactor 2 (HCF2), Protease inhibitor leuserpin-2 (HLS2), Heparin cofactor II (HC-II), which belongs to the serpin family. HCF2 is expressed predominantly in liver. Thrombin inhibitor activated by the glycosaminoglycans, heparin or dermatan sulfate. In the presence of the latter, SerpinD1/HC-II becomes the predominant thrombin inhibitor in place of antithrombin III (AT-III). In a glycosaminoglycan-independent manner, serpinD1/HC-II also inhibits chymotrypsin. Peptides at the N-terminal of HC-II have chemotactic activity for both monocytes and neutrophils.
• Predicted N Terminal: Gly20
• Form: Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 150 mM NaCl, pH 7.5, 10% trehalose.
• Molecular Mass: The protein has a calculated MW of 55.7 kDa. The protein migrates as 65-70 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
• Endotoxin: Less than 1.0 EU per μg by the LAL method.
• Purity: >95% as determined by SDS-PAGE.
• Storage: For long term storage, the product should be stored at lyophilized state at -20 centigrade or lower.
  Please avoid repeated freeze-thaw cycles.
  This product is stable after storage at:
  -20 to -70 centigrade for 12 months in lyophilized state;
  -70 centigrade for 3 months under sterile conditions after reconstitution.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4℃ before opening to recover the entire contents.

Gene Information

• Gene Name: SERPIND1
• Official Symbol: SERPIND1
• Synonyms: SERPIND1; serpin peptidase inhibitor, clade D (heparin cofactor), member 1; HCF2, serine (or cysteine) proteinase inhibitor, clade D (heparin cofactor), member 1; heparin cofactor 2; D22S673; HC II; HC2; heparin cofactor II; HLS2; serpin D1; leuserpin 2; protease inhibitor leuserpin-2; serine (or cysteine) proteinase inhibitor, clade D (heparin cofactor), member 1; LS2; HCF2; HCII; THPH10
• Gene ID: 3053
• mRNA Refseq: NM_000185
• Protein Refseq: NP_000176
• MIM: 142360
• UniProt ID: P05546