Recombinant Human SEMA3A, Fc-tagged

• Cat.No.: SEMA3A-12H
• Product Overview: A DNA sequence encoding the human SEMA3A (NP_006071.1) (Lys26-Phe546) was expressed with the Fc region of human IgG1 at the N-terminus.

Specification

• Description: Semaphorins are a family of secreted and cell-bound signaling molecules defined by the presence of a common 500 aa Sema domain. They are best characterized in relation to axon guidance during development of the nervous system. The functions of Semaphorins 3A (SEMA3A) are mediated primarily through binding to the Neuropilin-1 (Npn-1) and Plexin-A1 coreceptor complex. Neuropilins lack a signaling-competent cytoplasetmic domain and ensure semaphorin binding, whereas the transmembrane receptor plexin mediates the intracellular response. As the first identified vertebrate semaphorin, SEMA3A functions either as a chemorepulsive agent inhibiting axonal outgrowth, or as a chemoattractive agent stimulating the growth of apical dendrites. In both cases, the protein is vital for normal neuronal pattern development. Its overexpression is associated with schizophrenia which is seen in various human tumor cell lines, and aberrant release is associated with the progression of Alzheimer"s disease.
• Source: Human Cells
• Species: Human
• Tag: Fc
• Predicted N Terminal: Glu
• Form: Lyophilized from sterile PBS, pH 7.4.1. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.2. Please contact us for any concerns or special requirements.
• Molecular Mass: The recombinant human SEMA3A consists 781 amino acids and predicts a molecular mass of 87.7 kDa.
• Endotoxin: <1.0 eu per μg protein as determined by the lal</1.0
• Purity: >90 % as determined by SDS-PAGE.
• Stability: Samples are stable for up to twelve months from date of receipt at -70℃
• Storage: Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
• Protein length: 26-546 a.a.

Gene Information

• Gene Name: SEMA3A sema domain, immunoglobulin domain (Ig), short basic domain, secreted, (semaphorin) 3A [ Homo sapiens ]
• Official Symbol: SEMA3A
• Synonyms: HH16; SemD; COLL1; SEMA1; SEMAD; SEMAL; coll-1; Hsema-I; SEMAIII; Hsema-III; semaphorin-3A; collapsin 1; semaphorin D; semaphorin L; semaphorin III
• Gene ID: 10371
• mRNA Refseq: NM_006080
• Protein Refseq: NP_006071
• MIM: 603961
• UniProt ID: Q14563
• Chromosome Location: 7p12.1
• Pathway: Axon guidance, organism-specific biosystem; Axon guidance, conserved biosystem; Sema3A PAK dependent Axon repulsion, organism-specific biosystem
• Function: chemorepellent activity; neuropilin binding; receptor activity